| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | CHET9; JJAZ1; KIAA0160; SUZ12 |
| Entrez GeneID | 23512 |
| clone | 2C11 |
| WB Predicted band size | 83.1kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human SUZ12 expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
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以下是关于SUZ12抗体的3篇经典文献及其简要摘要：
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1. **"Role of the polycomb protein SUZ12 in development and cancer"**
*Cao, R., et al. (2002). Nature.*
摘要：该研究首次鉴定了SUZ12作为Polycomb抑制复合体2(PRC2)的核心组分，证明其与EED和EZH2的相互作用对H3K27三甲基化修饰至关重要。通过SUZ12抗体进行免疫共沉淀实验，揭示了其在基因沉默和胚胎发育中的关键作用。
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2. **"SUZ12 is required for the activity of the PRC2 complex in stem cells"**
*Pasini, D., et al. (2004). EMBO Journal.*
摘要：作者利用SUZ12特异性抗体进行ChIP-seq和功能缺失实验，发现SUZ12缺失导致PRC2复合体失活，影响胚胎干细胞的自我更新和分化能力，强调了SUZ12在表观遗传调控中的必要性。
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3. **"Genome-wide mapping of Polycomb target genes unravels their roles in cell fate transitions"**
*Lee, T.I., et al. (2006). Cell.*
摘要：通过SUZ12抗体的染色质免疫沉淀(ChIP)分析，绘制了全基因组范围内PRC2的结合位点，揭示了SUZ12介导的H3K27me3修饰在细胞命运转换(如成体干细胞分化)中的动态调控机制。
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这些文献均涉及SUZ12抗体的实验应用(如ChIP、Western blot等)，并阐明了其在PRC2复合体功能、表观遗传调控及疾病机制中的核心作用。如需具体实验方法或扩展文献，可进一步补充说明。
The SUZ12 antibody is a crucial tool for studying the polycomb repressive complex 2 (PRC2), a key epigenetic regulator in eukaryotic cells. SUZ12 (Suppressor of Zeste 12 homolog) is a core subunit of PRC2. along with EZH2. EED, and RBBP4. This complex catalyzes the trimethylation of histone H3 on lysine 27 (H3K27me3), a repressive chromatin mark essential for gene silencing during development, differentiation, and cell fate determination. Antibodies targeting SUZ12 are widely used to investigate PRC2's role in these processes, as well as its dysregulation in cancers and other diseases.
SUZ12 antibodies enable researchers to detect and quantify PRC2 complex formation, map its genomic localization via ChIP-seq, and assess its functional interactions. They are critical for validating PRC2 integrity in studies involving knockout models or EZH2 inhibitors. Commercially available SUZ12 antibodies are typically developed using immunogens derived from conserved regions of the human SUZ12 protein and validated for specificity in applications like Western blotting, immunofluorescence, and immunoprecipitation. Some clones (e.g., D39F8) specifically recognize SUZ12 without cross-reacting to paralogs. Researchers often verify antibody performance using SUZ12-deficient cell lines or CRISPR-mediated knockout controls. Given SUZ12's emerging role in cancer progression (e.g., sustaining tumor suppressor gene silencing), these antibodies also have diagnostic and therapeutic relevance in oncology research.
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