| WB | 1/500 - 1/2000 | Human,Mouse,Monkey |
| IF | 咨询技术 | Human,Mouse,Monkey |
| IHC | 1/200 - 1/1000 | Human,Mouse,Monkey |
| ICC | 技术咨询 | Human,Mouse,Monkey |
| FCM | 咨询技术 | Human,Mouse,Monkey |
| Elisa | 1/10000 | Human,Mouse,Monkey |
| Aliases | AIS1; HFH2; Genesis; FOXD3 |
| Entrez GeneID | 27022 |
| clone | 5G9 |
| WB Predicted band size | 50kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Monkey |
| Immunogen | Purified recombinant fragment of human FOXD3 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于FOXD3抗体的3篇参考文献及其摘要概括：
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1. **文献名称**: "The winged-helix transcription factor Foxd3 suppresses interneuron differentiation and promotes neural crest cell fate"
**作者**: Sutton et al.
**摘要**: 本研究利用FOXD3抗体进行免疫染色和功能分析，发现FOXD3在胚胎神经嵴细胞分化中起关键作用。抗体实验显示，FOXD3通过抑制神经元分化相关基因，维持神经嵴干细胞的未分化状态。
2. **文献名称**: "Foxd3 is a novel mediator of melanoma drug resistance and stemness"
**作者**: Yajima et al.
**摘要**: 通过FOXD3抗体的Western blot和免疫组化分析，作者发现FOXD3在黑色素瘤耐药细胞中高表达。研究揭示FOXD3通过调控干细胞相关通路，促进肿瘤耐药性和复发。
3. **文献名称**: "Foxd3 promotes pluripotency in embryonic stem cells by modulating the balance between self-renewal and differentiation"
**作者**: Hanna et al.
**摘要**: 该研究使用FOXD3抗体进行ChIP-seq和免疫荧光，证明FOXD3通过结合多能性基因(如Oct4)的启动子区，维持胚胎干细胞的自我更新能力，并抑制过早分化。
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以上文献均涉及FOXD3抗体的实验应用(如染色、蛋白检测、基因调控分析)，并聚焦于FOXD3在发育生物学及癌症中的功能机制。如需更多文献或具体实验细节，可进一步补充关键词或研究场景。
FOXD3 (Forkhead box D3) is a transcription factor belonging to the Forkhead family, characterized by a conserved forkhead DNA-binding domain. It plays critical roles in embryonic development, particularly in regulating neural crest cell differentiation, pluripotency maintenance in stem cells, and early embryogenesis. FOXD3 is also implicated in cancer biology, where it can act as both a tumor suppressor and promoter, depending on cellular context. In some cancers, it inhibits epithelial-mesenchymal transition (EMT) and metastasis, while in others, it promotes tumorigenesis by sustaining stem-like properties in cancer cells.
Antibodies targeting FOXD3 are essential tools for studying its expression, localization, and function. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). Researchers utilize these antibodies to investigate FOXD3's role in developmental biology, stem cell regulation, and cancer progression. Validation of FOXD3 antibodies is crucial due to potential cross-reactivity with other Forkhead family members. High-quality antibodies demonstrate specificity through knockdown/knockout controls and consistent detection across experimental models. Commercial FOXD3 antibodies are often raised against epitopes within its N-terminal region, with clones like 2A5 and E9 being commonly cited in literature.
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