| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPS6 |
| Uniprot No | P62753 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-249aa |
| 氨基酸序列 | MKLNISFPATGCQKLIEVDDERKLRTFYEKRMATEVAADALGEEWKGYVV RISGGNDKQGFPMKQGVLTHGRVRLLLSKGHSCYRPRRTGERKRKSVRGC IVDANLSVLNLVIVKKGEKDIPGLTDTTVPRRLGPKRASRIRKLFNLSKE DDVRQYVVRKPLNKEGKKPRTKAPKIQRLVTPRVLQHKRRRIALKKQRTK KNKEEAAEYAKLLAKRMKEAKEKRQEQIAKRRRLSSLRASTSKSESSQK |
| 预测分子量 | 53 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与RPS6重组蛋白相关的文献概览：
1. **文献名称**：*Regulation of Ribosomal Protein S6 Phosphorylation by the mTOR Signaling Pathway*
**作者**：Meyuhas O., et al.
**摘要**：研究mTOR通路对RPS6磷酸化的调控机制，利用重组RPS6蛋白进行体外激酶实验，揭示其在细胞生长和蛋白质合成中的关键作用。
2. **文献名称**：*Expression and Purification of Recombinant Human RPS6 for Structural Studies*
**作者**：Johnson R., Smith T.
**摘要**：报道人源RPS6重组蛋白在大肠杆菌中的高效表达与纯化方法，通过X射线晶体学解析其三维结构，为靶向RPS6的药物设计提供基础。
3. **文献名称**：*RPS6 Knockdown and Recombinant Protein Rescue in Cancer Cell Models*
**作者**：Chen L., et al.
**摘要**：通过siRNA敲低RPS6抑制肿瘤细胞增殖，并利用重组RPS6蛋白回补实验验证其功能，表明RPS6在癌症代谢中的潜在治疗靶点价值。
注：以上为示例性内容，实际文献需通过学术数据库(如PubMed/Web of Science)检索确认。
**Background of Recombinant RPS6 Protein**
Ribosomal protein S6 (RPS6) is a key component of the 40S ribosomal subunit, playing a critical role in protein synthesis and cellular homeostasis. It is involved in regulating ribosome biogenesis, mRNA translation, and cellular responses to stress or growth signals. RPS6 is notably phosphorylated in response to mitogenic or nutrient-sensing pathways, particularly the mTOR (mechanistic target of rapamycin) signaling pathway, which links its activity to cell proliferation, metabolism, and survival. Dysregulation of RPS6 or its phosphorylation has been implicated in various diseases, including cancer, diabetes, and neurodegenerative disorders.
Recombinant RPS6 protein is engineered through molecular cloning techniques, typically expressed in bacterial (e.g., *E. coli*) or eukaryotic systems (e.g., mammalian cells) to ensure proper post-translational modifications, such as phosphorylation. Its production enables detailed biochemical and functional studies, including interactions with kinases (e.g., S6K), phosphatases, or ribosomal components. Researchers use recombinant RPS6 to investigate its role in translation regulation, ribosome assembly, and disease mechanisms. Additionally, it serves as a tool for drug screening, particularly for therapies targeting mTOR-related pathways.
The development of recombinant RPS6 has advanced understanding of ribosome function and cellular signaling networks. Challenges remain in mimicking native phosphorylation states *in vitro*, but ongoing innovations in protein engineering and expression systems continue to enhance its utility in both basic research and therapeutic applications.
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