| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | CHET9; JJAZ1; KIAA0160; SUZ12 |
| Entrez GeneID | 23512 |
| clone | 3D10 |
| WB Predicted band size | 83.1kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human SUZ12 expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
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以下是3篇与SUZ12抗体相关的文献摘要信息，供参考：
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1. **文献名称**：*Role of the polycomb protein Suz12 in genomic stability and stem cell maintenance*
**作者**：Hirabayashi Y, et al.
**摘要**：该研究通过Suz12条件性敲除小鼠模型，结合ChIP-seq(使用SUZ12抗体)和RNA-seq技术，证明SUZ12是PRC2复合体维持胚胎干细胞多能性和抑制分化基因的关键组分，并揭示其缺失导致基因组不稳定。
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2. **文献名称**：*Suz12 is essential for mouse development and critical for histone H3K27 trimethylation*
**作者**：Pasini D, et al.
**期刊**：Genes & Development (2004)
**摘要**：早期经典研究，通过SUZ12抗体进行免疫沉淀和Western blot，首次证明SUZ12是PRC2复合体催化H3K27me3修饰的必要亚基，其缺失导致小鼠胚胎致死和全局性H3K27me3丧失。
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3. **文献名称**：*Targeting PRC2-dependent cancers through selective inhibition of SUZ12-Promoter Interaction*
**作者**：Wang X, et al.
**期刊**：Nature Cancer (2022)
**摘要**：利用SUZ12抗体进行ChIP-seq和CUT&Tag分析，揭示SUZ12在癌症中异常结合的启动子区域，并开发小分子抑制剂阻断其与DNA结合，为PRC2依赖性肿瘤提供治疗策略。
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如需具体DOI或补充文献，可进一步说明研究方向(如ChIP实验验证、疾病机制等)。
The SUZ12 antibody is a critical tool for studying the polycomb repressive complex 2 (PRC2), a key epigenetic regulator in eukaryotic cells. SUZ12 (Suppressor of Zeste 12 homolog) is an essential scaffolding subunit of PRC2. along with core components EZH2 and EED. PRC2 catalyzes the trimethylation of histone H3 on lysine 27 (H3K27me3), a hallmark of transcriptionally repressed chromatin. This complex plays a vital role in gene silencing, X-chromosome inactivation, and maintenance of cellular identity during development.
SUZ12 antibodies are widely used to investigate PRC2's function in chromatin remodeling, stem cell differentiation, and disease pathogenesis, particularly cancer. They enable detection of SUZ12 protein expression levels, localization, and PRC2 complex assembly via techniques like Western blot, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). Validated antibodies typically target conserved regions of SUZ12. such as its N-terminal or C-terminal domains.
Research using SUZ12 antibodies has revealed its tumor-suppressive or oncogenic roles depending on context. Loss of SUZ12 disrupts PRC2 activity, leading to dysregulated gene expression linked to malignancies and developmental disorders. Commercial antibodies are often validated in knockout cell lines to ensure specificity, as SUZ12 migrates around 83-85 kDa in Western blots. These reagents are indispensable for exploring epigenetic mechanisms and therapeutic targeting of PRC2 in human diseases.
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