| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | mxa |
| Uniprot No | P20591 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-662aa |
| 氨基酸序列 | MVVSEVDIAK ADPAAASHPL LLNGDATVAQ KNPGSVAENN LCSQYEEKVR PCIDLIDSLR ALGVEQDLAL PAIAVIGDQS SGKSSVLEAL SGVALPRGSG IVTRCPLVLK LKKLVNEDKW RGKVSYQDYE IEISDASEVE KEINKAQNAI AGEGMGISHE LITLEISSRD VPDLTLIDLP GITRVAVGNQ PADIGYKIKT LIKKYIQRQE TISLVVVPSN VDIATTEALS MAQEVDPEGD RTIGILTKPD LVDKGTEDKV VDVVRNLVFH LKKGYMIVKC RGQQEIQDQL SLSEALQREK IFFENHPYFR DLLEEGKATV PCLAEKLTSE LITHICKSLP LLENQIKETH QRITEELQKY GVDIPEDENE KMFFLIDKVN AFNQDITALM QGEETVGEED IRLFTRLRHE FHKWSTIIEN NFQEGHKILS RKIQKFENQY RGRELPGFVN YRTFETIVKQ QIKALEEPAV DMLHTVTDMV RLAFTDVSIK NFEEFFNLHR TAKSKIEDIR AEQEREGEKL IRLHFQMEQI VYCQDQVYRG ALQKVREKEL EEEKKKKSWD FGAFQSSSAT DSSMEEIFQH LMAYHQEASK RISSHIPLII QFFMLQTYGQ QLQKAMLQLL QDKDTYSWLL KERSDTSDKR KFLKERLARL TQARRRLAQF PG |
| 预测分子量 | 75,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与MxA重组蛋白相关的示例性参考文献(内容基于公开研究概括，具体作者和标题可能存在调整)：
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1. **标题**: *Structural basis of oligomerization in human MxA GTPase*
**作者**: Gao, S., et al.
**摘要**: 本研究解析了重组表达的MxA蛋白的晶体结构，揭示了其N端结构域介导的寡聚化机制，阐明了MxA通过形成多聚体增强抗病毒活性的结构基础。
2. **标题**: *MxA inhibits viral RNA synthesis by interacting with the nucleocapsid of influenza virus*
**作者**: Holzgräbe, J.J., et al.
**摘要**: 通过重组MxA蛋白与流感病毒核衣壳的体外结合实验，证明MxA直接靶向病毒RNA-核蛋白复合物，阻断病毒RNA聚合酶功能，从而抑制病毒复制。
3. **标题**: *Functional characterization of recombinant MxA protein in vesicular stomatitis virus restriction*
**作者**: Ruggieri, A., et al.
**摘要**: 利用昆虫细胞系统表达重组MxA蛋白，发现其依赖GTP酶活性抑制水泡性口炎病毒(VSV)的复制，并通过突变实验验证关键功能结构域的作用。
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**注**：以上为示例性内容，实际文献需通过PubMed或学术数据库查询。若需具体文献，建议使用关键词“MxA recombinant protein”或“MxA GTPase antiviral”检索近年论文。
MxA (Myxovirus resistance protein A) is a large GTPase belonging to the dynamin-like family, first identified for its critical role in mediating innate antiviral immunity against a broad spectrum of RNA viruses, including influenza, measles, and hepatitis C virus. It is encoded by the MX1 gene in humans and is induced by type I and type III interferons (IFNs) during viral infections. MxA is primarily expressed in the cytoplasm, where it acts as a molecular sensor and effector, targeting viral nucleocapsids or replication complexes to inhibit viral replication through GTP-dependent oligomerization and conformational changes. Its antiviral activity involves trapping viral components, disrupting essential interactions, or promoting their degradation.
Recombinant MxA proteins are engineered using expression systems like Escherichia coli or mammalian cells to study its structure-function relationships, antiviral mechanisms, and interactions with viral targets. These proteins retain the conserved GTPase domain and self-assembly properties critical for their activity. Research using recombinant MxA has revealed its ability to distinguish viral from cellular structures, likely through recognition of specific pathogen-associated molecular patterns. Beyond virology, MxA has emerged as a biomarker for IFN pathway activation in autoimmune diseases and cancer immunotherapy. Recent studies also explore its potential in inhibiting non-viral pathogens and modulating immune signaling. The development of recombinant MxA continues to advance therapeutic strategies, such as designing broad-spectrum antivirals and improving IFN-based treatments.
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