| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | ETS-1; EWSR2; FLJ10768; ETS1 |
| Entrez GeneID | 2113 |
| clone | 8A8 |
| WB Predicted band size | 50.4kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human ETS1 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ETS1抗体的3篇示例参考文献(内容为模拟，非真实文献)：
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1. **文献名称**: "ETS1 transcription factor regulates immune cell development and autoimmunity"
**作者**: Smith A, et al.
**摘要**: 研究利用ETS1特异性抗体分析其在T/B细胞分化中的作用，发现ETS1缺失导致自身免疫反应增强，揭示其在维持免疫耐受中的关键调控机制。
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2. **文献名称**: "Prognostic significance of ETS1 overexpression in breast cancer via antibody-based detection"
**作者**: Chen L, et al.
**摘要**: 通过免疫组化(ETS1抗体)评估乳腺癌患者样本，证实ETS1高表达与肿瘤转移和不良预后相关，提示其作为潜在生物标志物的价值。
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3. **文献名称**: "ETS1 antibody-based chromatin profiling reveals target genes in endothelial cells"
**作者**: Kim J, et al.
**摘要**: 采用ChIP-seq技术结合ETS1抗体，鉴定出血管内皮细胞中ETS1直接调控的靶基因网络，阐明其在血管生成和炎症反应中的双重功能。
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注：以上文献为示例模板，实际引用时需通过PubMed/Google Scholar检索真实研究，关键词建议：**ETS1 antibody** + **immune/cancer/ChIP**。
The ETS1 antibody is a crucial tool in molecular and cellular biology research, targeting the ETS1 protein, a member of the ETS (E-twenty-six) family of transcription factors. ETS1 regulates gene expression by binding to DNA sequences containing a conserved GGAA/T motif, playing roles in cell differentiation, apoptosis, angiogenesis, and immune response. Dysregulation of ETS1 is linked to cancer progression, autoimmune diseases, and developmental disorders.
The antibody is widely used to detect ETS1 expression and localization in tissues or cultured cells via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). It aids in studying ETS1's functional mechanisms, including its interaction with co-regulators or response to signaling pathways (e.g., MAPK). Researchers also utilize it to explore ETS1's role in tumor invasiveness, lymphocyte development, and endothelial cell behavior.
Commercial ETS1 antibodies are typically raised in rabbits or mice, targeting specific epitopes (e.g., N-terminal or C-terminal regions). Validation includes testing for specificity using knockout controls or siRNA-mediated knockdown. Challenges may arise due to homology among ETS family members, necessitating careful optimization. Its applications span oncology, immunology, and developmental biology, making it a vital reagent for understanding ETS1-mediated transcriptional regulation in health and disease.
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