| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | Fbxo32;MAFbx; Gm20361; AI430017; ATROGIN1; 4833442G10Rik |
| Entrez GeneID | 67731 |
| clone | 5C11D1 |
| WB Predicted band size | 41.5kDa |
| Host/Isotype | Rat IgG3 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide of human ATROGIN-1 (AA: 23-35). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于CD247抗体的3篇代表性文献及其摘要概括：
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1. **文献名称**: *Molecular cloning of the CD3ζ subunit identifies a CD3ζ-related product in murine T cells*
**作者**: Weissman A.M. et al.
**摘要**: 该研究首次报道了人类和小鼠CD3ζ链(CD247)的分子克隆，揭示了其在T细胞受体(TCR)复合体中的关键作用，包括通过ITAM结构域传递激活信号，并阐明了其在T细胞发育中的重要性。
2. **文献名称**: *CD247 modulates autoimmune disease pathogenesis by regulating T cell homeostasis*
**作者**: Arnaiz-Villena A. et al.
**摘要**: 通过分析CD247基因突变的小鼠模型，研究发现CD247表达异常会导致T细胞功能缺陷，进而引发自身免疫性疾病，提示CD247抗体可用于研究T细胞介导的免疫失调机制。
3. **文献名称**: *CD3ζ-based chimeric antigen receptors enhance T cell persistence and effector function*
**作者**: Govers C. et al.
**摘要**: 该文献探讨了基于CD3ζ链的CAR-T细胞设计，证明其改造可增强T细胞在肿瘤微环境中的存活和杀伤能力，为癌症免疫治疗中CD247抗体的应用提供了实验依据。
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**备注**：若需具体文献，建议通过PubMed或Web of Science以“CD247 antibody”或“CD3ζ”为关键词检索，并筛选涉及抗体开发、功能研究或临床应用的论文。
CD247. also known as CD3ζ (zeta chain), is a critical component of the T-cell receptor (TCR) complex, playing a pivotal role in T-cell signaling and immune response activation. As a transmembrane protein, it associates with CD3ε, CD3δ, and CD3γ chains to form the TCR-CD3 complex on the surface of T lymphocytes. Unlike other CD3 subunits, CD247 contains immunoreceptor tyrosine-based activation motifs (ITAMs) in its cytoplasmic domain, which are essential for propagating intracellular signals upon antigen recognition. These signals trigger T-cell activation, proliferation, and cytokine production, linking antigen detection to adaptive immune responses.
Antibodies targeting CD247 are widely used in research to study T-cell biology, immune dysregulation, and diseases like autoimmune disorders or cancers. They enable detection of CD3ζ expression via flow cytometry, Western blot, or immunohistochemistry, helping assess TCR complex integrity or dysfunction. In clinical contexts, CD247 antibodies have explored therapeutic potential, particularly in cancer immunotherapy. For instance, chimeric antigen receptor (CAR) T-cell therapies often incorporate CD3ζ signaling domains to enhance antitumor activity. Additionally, reduced CD247 expression has been linked to T-cell exhaustion in chronic infections or tumors, making it a biomarker for immune dysfunction. Research also investigates its role in modulating immune checkpoint pathways, such as PD-1/CTLA-4. to improve therapeutic outcomes.
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