| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | ihfB |
| Uniprot No | P0A6Y1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-94aa |
| 氨基酸序列 | MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIEIRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG |
| 预测分子量 | 10,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ihfB重组蛋白的3篇参考文献，简要概括其内容：
1. **文献名称**: "Cloning, expression, and purification of the ihfB gene product of Escherichia coli"
**作者**: Smith J, et al.
**摘要**: 该研究成功将大肠杆菌ihfB基因克隆至表达载体，通过IPTG诱导表达重组蛋白，并利用亲和层析技术纯化。实验验证了重组IHFB蛋白的DNA结合活性，证实其功能完整性。
2. **文献名称**: "Functional analysis of recombinant IHFB in bacterial pathogenesis"
**作者**: Lee S, et al.
**摘要**: 研究在大肠杆菌中表达并纯化IHFB重组蛋白，探讨其在病原菌毒力调控中的作用。通过凝胶迁移实验(EMSA)证明IHFB与特定启动子区域结合，影响毒力基因表达。
3. **文献名称**: "Structural insights into the DNA-binding mechanism of recombinant IHFB subunit"
**作者**: Zhang Y, et al.
**摘要**: 利用X射线晶体学解析重组IHFB蛋白的三维结构，揭示其通过特定α螺旋与DNA小沟结合的分子机制，并发现关键氨基酸突变会显著削弱结合能力。
4. **文献名称**: "Heterologous expression of ihfB in Pseudomonas putida for synthetic biology applications"
**作者**: Kumar R, et al.
**摘要**: 研究在恶臭假单胞菌中异源表达ihfB重组蛋白，优化表达条件后证明其可增强外源基因回路稳定性，为合成生物学工具开发提供支持。
*注：以上文献为示例性内容，实际引用时建议通过数据库(如PubMed、Web of Science)核对具体信息。*
The integration host factor (IHF) is a nucleoid-associated protein in prokaryotes, composed of two subunits, IhfA (α) and IhfB (β), encoded by the ihfA and ihfB genes, respectively. IHF plays a critical role in DNA compaction, replication, recombination, and transcriptional regulation by binding to specific DNA sequences and inducing sharp bends, facilitating interactions between distant genomic regions or between DNA and regulatory proteins. The ihfB gene product, the β-subunit, is essential for forming the functional IHF heterodimer.
Recombinant IhfB protein is produced via genetic engineering, typically by cloning the ihfB gene into expression vectors (e.g., plasmids) and overexpressing it in heterologous hosts like *E. coli*. This allows large-scale purification of the protein for biochemical and structural studies. Recombinant IhfB is often tagged (e.g., His-tag) to simplify purification via affinity chromatography. Its production has enabled detailed analyses of IHF’s DNA-binding specificity, conformational effects on DNA, and roles in bacterial processes such as virulence, biofilm formation, and phage integration.
Studies using recombinant IhfB have also clarified its interactions with partner proteins (e.g., IhfA) and its contribution to stress responses. In biotechnology, IhfB is utilized in synthetic biology for designing DNA scaffolds or modulating gene circuits. Despite its prokaryotic origin, insights from IhfB have broader implications for understanding chromatin organization and gene regulation mechanisms across organisms.
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