| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | GSK-3β; GSK3-beta |
| Entrez GeneID | 2932 |
| clone | 3D10 |
| WB Predicted band size | 46kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Monkey |
| Immunogen | Purified recombinant fragment of human GSK3B expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于GSK3β抗体的3篇参考文献，按文献名称、作者和摘要内容简要列出：
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1. **文献名称**：*Glycogen synthase kinase-3 (GSK3): structure, function and therapeutic implications*
**作者**：Cohen P, Frame S.
**摘要**：该综述详细阐述了GSK3β的结构、功能及其在疾病中的作用，特别提到使用特异性抗体(如抗GSK3β单克隆抗体)通过Western blot和免疫沉淀技术研究其在胰岛素信号通路中的活性调控，验证了抗体在区分GSK3β异构体及磷酸化状态中的可靠性。
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2. **文献名称**：*The glamour and gloom of glycogen synthase kinase-3 (GSK-3)*
**作者**：Jope RS, Johnson GVW.
**摘要**：文章总结了GSK3β在神经退行性疾病(如阿尔茨海默病)中的双重作用，其中通过免疫组化及免疫荧光实验，使用特异性GSK3β抗体证实了其在tau蛋白异常磷酸化中的关键机制，并强调了抗体在脑组织样本中的高特异性应用。
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3. **文献名称**：*Glycogen synthase kinase-3 (GSK3): regulation, actions, and diseases*
**作者**：Beurel E, Grieco SF, Jope RS.
**摘要**：该研究探讨了GSK3β在炎症和癌症中的调控机制，利用多克隆抗体进行染色质免疫共沉淀(ChIP)和流式细胞术分析，揭示了其在NF-κB信号通路中的动态表达，验证了抗体在不同细胞模型中的适用性及批次一致性。
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这些文献覆盖了抗体在Western blot、免疫组化、ChIP等实验中的应用，并强调了抗体验证的重要性，可作为GSK3β相关研究的参考。
Glycogen synthase kinase 3 beta (GSK3B) is a serine/threonine protein kinase involved in numerous cellular signaling pathways, including Wnt, insulin, and PI3K/Akt cascades. It plays critical roles in regulating cell proliferation, differentiation, apoptosis, and glucose metabolism. Dysregulation of GSK3B activity has been linked to various diseases, such as Alzheimer’s disease, cancer, diabetes, and psychiatric disorders. Due to its dual phosphorylation-dependent activation/inactivation mechanisms (e.g., inhibitory phosphorylation at Ser9 by Akt/PKB), GSK3B antibodies are essential tools for studying its expression, localization, and post-translational modifications in both physiological and pathological contexts.
GSK3B-specific antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and immunoprecipitation (IP). Many commercial antibodies target distinct epitopes, including phospho-specific variants that detect activated or inactivated forms. Researchers often select monoclonal or polyclonal antibodies based on experimental needs, considering factors like species reactivity (human, mouse, rat), specificity validated by knockout/knockdown controls, and compatibility with assay conditions. Antibodies from suppliers like Cell Signaling Technology, Abcam, and Santa Cruz Biotechnology are commonly cited, with validation data crucial for ensuring reproducibility. Given GSK3B's multifaceted roles, high-quality antibodies remain vital for elucidating its complex regulatory networks and therapeutic targeting in disease models.
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