| WB | 1/500 - 1/2000 | Human,Rat,Monkey |
| IF | 咨询技术 | Human,Rat,Monkey |
| IHC | 1/200 - 1/1000 | Human,Rat,Monkey |
| ICC | 1/200 - 1/1000 | Human,Rat,Monkey |
| FCM | 咨询技术 | Human,Rat,Monkey |
| Elisa | 1/10000 | Human,Rat,Monkey |
| Aliases | A630002H24; B-and T-lymphocyte-associated protein |
| Entrez GeneID | 208154 |
| WB Predicted band size | Calculated MW: 34 kDa; Observed MW: 55-80 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Recombinant protein of mouse CD272 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于AKT2抗体的3篇参考文献示例(注：文献为假设性示例，实际引用需查询真实数据库)：
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1. **文献名称**：*"Selective AKT2 Inhibition in Adipose Tissue Suppresses Lipid Accumulation"*
**作者**：Zhang, L. et al.
**摘要**：本研究利用特异性AKT2抗体，通过Western blot和免疫荧光技术，证实抑制AKT2可显著减少脂肪细胞中的脂质沉积，为代谢综合征治疗提供新靶点。
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2. **文献名称**：*"AKT2-Specific Antibody Reveals Its Role in Ovarian Cancer Metastasis"*
**作者**：Gupta, R. & Thompson, D.
**摘要**：通过高特异性AKT2抗体进行组织芯片分析，发现AKT2在卵巢癌转移灶中高表达，并与患者生存率负相关，提示其作为预后标志物的潜力。
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3. **文献名称**：*"Development of a Phospho-AKT2 (Ser474) Monoclonal Antibody for Functional Studies"*
**作者**：Kato, H. et al.
**摘要**：报道了一种针对AKT2 Ser474磷酸化位点的新型单克隆抗体的开发与验证，成功应用于检测胰岛素刺激下肌肉细胞中AKT2的激活动态。
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如需真实文献，建议通过PubMed或Google Scholar检索关键词“AKT2 antibody”、“AKT2 detection”或结合具体研究领域(如癌症、代谢疾病)进一步筛选。
The AKT2 antibody is a crucial tool in studying the protein kinase B (PKB) beta isoform, encoded by the *AKT2* gene. As a member of the AKT serine/threonine kinase family, AKT2 plays a central role in the PI3K/AKT/mTOR signaling pathway, which regulates critical cellular processes such as survival, proliferation, glucose metabolism, and apoptosis. Unlike its isoforms AKT1 and AKT3. AKT2 is particularly associated with insulin signaling and glucose homeostasis, making it a focus in metabolic disorders like diabetes and insulin resistance. Dysregulation of AKT2 is also implicated in cancer, where its overexpression or activation contributes to tumor growth, metastasis, and chemoresistance in malignancies like ovarian, pancreatic, and breast cancers.
AKT2 antibodies are designed to detect and quantify AKT2 protein expression, phosphorylation states (e.g., at Ser474 or Thr309/314), or specific isoforms in biological samples. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. Researchers rely on them to explore AKT2’s role in disease mechanisms, validate gene-editing outcomes, or assess therapeutic responses. Specificity is critical, as cross-reactivity with AKT1/AKT3 can occur; thus, antibodies are often validated using knockout controls. Commercial AKT2 antibodies vary in clonality, epitope targets, and applications, necessitating careful selection based on experimental needs. Their utility extends to drug development, particularly in targeting AKT2 for cancer therapy or metabolic disease treatment.
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