| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NAV1 |
| Uniprot No | Q8NEY1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1362-1492aa |
| 氨基酸序列 | APGPSSGSTPGQVPGSSALSSPRRSLGLALTHSFGPSLADTDLSPMDGISTCGPKEEVTLRVVVRMPPQHIIKGDLKQQEFFLGCSKVSGKVDWKMLDEAVFQVFKDYISKMDPASTLGLSTESIHGYSIS |
| 预测分子量 | 18.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NAV1重组蛋白的3篇参考文献示例：
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1. **文献名称**：*"Expression and Purification of Recombinant Voltage-Gated Sodium Channel Nav1.7 in HEK293 Cells"*
**作者**：Li, X. et al.
**摘要**：研究报道了在HEK293细胞中成功表达并纯化人源Nav1.7重组蛋白，通过电生理实验验证其功能活性，为药物筛选提供了工具。
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2. **文献名称**：*"Structural Insights into Nav1.5 Using Cryo-EM: Implications for Cardiac Arrhythmia"*
**作者**：Chen, J. & Huang, C.L.
**摘要**：利用冷冻电镜技术解析了重组Nav1.5蛋白的高分辨率结构，揭示其门控机制，为治疗心律失常的靶向药物设计提供依据。
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3. **文献名称**：*"Functional Characterization of Recombinant Nav1.2 Channels in a Xenopus Oocyte System"*
**作者**：Wang, Y. et al.
**摘要**：通过在非洲爪蟾卵母细胞中表达重组Nav1.2蛋白，分析了其电生理特性及突变体对通道功能的影响，探讨了其在癫痫中的作用。
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注：上述文献为示例，实际引用时请核实具体论文信息及数据库来源(如PubMed、Nature等)。若需特定亚型(如Nav1.1-1.9)的研究，可进一步调整关键词检索。
**Background of NAV1 Recombinant Protein**
NAV1. a voltage-gated sodium channel protein, plays a critical role in generating and propagating action potentials in excitable cells, including neurons and muscle cells. As a member of the sodium channel family, NAV1 (also designated as SCNx based on genetic nomenclature) is characterized by its α-subunit, which forms the core pore for sodium ion conductance. Dysregulation of NAV1 channels is linked to neurological disorders, cardiac arrhythmias, and chronic pain syndromes, making them significant therapeutic targets.
Recombinant NAV1 protein is engineered using heterologous expression systems, such as mammalian (e.g., HEK293 or CHO cells) or insect cell lines (e.g., Sf9), to ensure proper post-translational modifications and functional folding. The recombinant protein typically retains key structural domains, including voltage-sensing segments, pore-forming regions, and inactivation gates, enabling its use in electrophysiological studies, drug screening, and structural biology.
Research applications of NAV1 recombinant protein include elucidating channelopathies, investigating toxin interactions (e.g., tetrodotoxin), and developing sodium channel-blocking drugs for pain management or antiarrhythmic therapies. Its production often incorporates tags (e.g., His-tag or GFP) for purification or visualization. Challenges remain in maintaining stability and full functionality *in vitro*, driving advances in membrane protein solubilization and cryo-EM structural analysis.
Overall, NAV1 recombinant protein serves as a vital tool for dissecting sodium channel physiology and accelerating precision medicine for related diseases.
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