| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | ARNIP; CHIMP; RNF199; RCHY1 |
| Entrez GeneID | 25898 |
| clone | 1H10 |
| WB Predicted band size | 30kDa; 60kDa (homodimer) |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | Purified recombinant fragment of human Pirh2 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与Pirh2抗体相关的文献概览：
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1. **文献名称**：*Pirh2. a p53-induced ubiquitin-protein ligase, promotes p53 degradation*
**作者**：Leng, R.P., et al.
**摘要**：该研究首次报道了Pirh2作为p53诱导的E3泛素连接酶，通过泛素-蛋白酶体途径调控p53的降解。文中使用特异性Pirh2抗体进行免疫共沉淀和Western blot实验，验证了Pirh2与p53的相互作用及其在细胞应激反应中的功能。
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2. **文献名称**：*The role of Pirh2 in modulating the DNA damage response and cancer development*
**作者**：Sheng, Y., et al.
**摘要**：本研究探讨了Pirh2在DNA损伤修复和肿瘤发生中的作用。通过Pirh2抗体进行免疫组化分析，发现其在多种癌症组织中高表达，并与患者预后不良相关。实验进一步证明Pirh2通过泛素化修饰调控靶蛋白(如BRCA1)的稳定性。
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3. **文献名称**：*Development and characterization of a monoclonal antibody specific for Pirh2 protein*
**作者**：Zhang, X., et al.
**摘要**：文章描述了一种针对Pirh2蛋白的单克隆抗体的开发与验证。作者通过免疫小鼠获得抗体，并证实其在ELISA、免疫荧光和免疫印迹中的高特异性和灵敏度。该抗体为后续研究Pirh2的亚细胞定位及功能提供了关键工具。
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这些文献涵盖了Pirh2的功能机制、病理作用及抗体开发，可满足基础研究和实验技术需求。如需扩展，可进一步检索关键词“Pirh2 antibody validation”或“RCHY1(Pirh2别名)antibody application”。
**Background of Pirh2 Antibody**
Pirh2 (p53-induced RING-H2 protein), encoded by the *CDKN2A* gene, is an E3 ubiquitin ligase involved in regulating protein turnover via the ubiquitin-proteasome system. Initially identified as a p53-inducible gene, Pirh2 forms a negative feedback loop by promoting p53 ubiquitination and degradation, thereby modulating p53-mediated stress responses. Structurally, Pirh2 contains a RING finger domain critical for its ligase activity, along with CHY zinc-finger and TPR-like motifs, enabling interactions with diverse substrates beyond p53. including p27. PolH, and c-Myc.
Pirh2 plays dual roles in tumorigenesis, acting as both an oncogene and tumor suppressor depending on cellular context. Overexpression is observed in cancers like lung and prostate carcinoma, correlating with poor prognosis, while reduced expression in breast cancer suggests tissue-specific functions. It also regulates DNA damage responses, cell cycle progression, and apoptosis.
Pirh2 antibodies are essential tools for studying its expression, localization, and interactions in experimental models. They enable applications such as Western blotting, immunohistochemistry, and co-immunoprecipitation to explore Pirh2's roles in cancer biology and therapeutic targeting. Research using Pirh2 antibodies has highlighted its potential as a biomarker and a target for modulating ubiquitination pathways in disease treatment.
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