| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | HD4; HDACA; HA6116; HDAC-A; KIAA0288; HDAC4 |
| Entrez GeneID | 9759 |
| clone | 7B2 |
| WB Predicted band size | 119kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human HDAC4 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于HDAC4抗体的3篇参考文献示例(注：内容为模拟概括，实际文献需根据具体数据库检索)：
1. **文献名称**：*HDAC4 regulates neuronal survival in Huntington’s disease*
**作者**：M. Majdzadeh et al.
**摘要**：研究利用HDAC4特异性抗体，通过免疫印迹和免疫荧光技术，发现HDAC4在亨廷顿病模型中的核质穿梭异常，其乙酰化状态与神经元存活密切相关。
2. **文献名称**：*Selective inhibition of HDAC4 in cardiac hypertrophy*
**作者**：J. Backs et al.
**摘要**：通过HDAC4抗体检测心脏组织中的蛋白表达，揭示HDAC4在病理性心肌肥厚中的调控作用，抑制其活性可缓解心脏功能障碍。
3. **文献名称**：*HDAC4 deacetylation mediates muscle atrophy*
**作者**：S. Cohen et al.
**摘要**：研究使用HDAC4抗体进行染色质免疫沉淀(ChIP)，证明HDAC4通过去乙酰化FoxO转录因子促进肌肉萎缩相关基因表达。
4. **文献名称**：*Antibody-based profiling of HDAC4 isoforms in cancer*
**作者**：K. Ishii et al.
**摘要**：开发了一种高特异性HDAC4抗体，用于区分不同剪接变体，并发现特定异构体在乳腺癌中的异常表达与预后相关。
(注：以上为示例性内容，实际文献请通过PubMed或Google Scholar检索确认。)
**Background of HDAC4 Antibody**
Histone deacetylase 4 (HDAC4) is a member of the class IIa HDAC family, which plays a critical role in epigenetic regulation by removing acetyl groups from histones, leading to chromatin condensation and transcriptional repression. HDAC4 is involved in diverse cellular processes, including muscle differentiation, bone development, neuronal survival, and stress responses. It shuttles between the nucleus and cytoplasm, interacting with transcription factors like MEF2 to modulate gene expression. Dysregulation of HDAC4 has been linked to cancers, neurodegenerative diseases, and cardiovascular disorders, making it a potential therapeutic target.
HDAC4 antibodies are essential tools for studying its expression, localization, and function. They are widely used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and co-immunoprecipitation (Co-IP). These antibodies typically target specific epitopes or post-translational modification sites (e.g., phosphorylation sites), aiding in the analysis of HDAC4’s regulatory mechanisms. Validated HDAC4 antibodies ensure specificity across species, often cross-reacting with human, mouse, and rat samples. Researchers rely on these antibodies to explore HDAC4’s role in disease pathways or its interaction with signaling molecules, providing insights into targeted therapies. Proper controls, such as knockout cell lines, are recommended to confirm antibody specificity in experimental settings.
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