| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | RY; APG-2; hsp70; hsp70RY; HS24/P52; MGC131852; HSPA4 |
| Entrez GeneID | 3308 |
| clone | 5A6 |
| WB Predicted band size | 70kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human HSP70 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于HSP70抗体的3篇代表性文献摘要(示例格式，非真实文献)：
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1. **文献名称**: *"Heat shock proteins: Stress proteins with essential functions in cellular homeostasis"*
**作者**: Welch WJ, Morimoto RI
**摘要**: 该综述系统阐述了HSP70家族在细胞应激反应中的核心作用，包括其作为分子伴侣参与蛋白质折叠、修复错误折叠蛋白的功能，以及在热休克、氧化应激等条件下的表达调控机制。
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2. **文献名称**: *"HSP70 overexpression inhibits apoptosis in human cancer cells by targeting multiple signaling pathways"*
**作者**: Jaattela M
**摘要**: 研究通过HSP70抗体检测发现，HSP70在多种癌细胞中高表达，并通过抑制线粒体凋亡通路和阻断caspase活化增强癌细胞对化疗的抵抗，提示其作为癌症治疗靶点的潜力。
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3. **文献名称**: *"Antibody-based detection of HSP70 in neurodegenerative disease models"*
**作者**: Turturici G et al.
**摘要**: 利用特异性HSP70抗体在小鼠阿尔茨海默病模型中发现，HSP70在神经元中通过减少tau蛋白聚集和抑制神经炎症发挥保护作用，为神经退行性疾病治疗提供新思路。
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4. **文献名称**: *"HSP70 as a biomarker for tumor diagnosis and prognosis"*
**作者**: Dutta SK et al.
**摘要**: 通过免疫组化分析HSP70抗体在多种实体瘤中的表达水平，发现其与肿瘤分期、转移及患者生存率显著相关，提出HSP70可作为临床肿瘤诊断和预后评估的生物标志物。
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注：以上为示例性内容，实际文献需通过PubMed或Google Scholar检索获取。
HSP70 antibodies are essential tools for studying the 70-kDa heat shock protein family, a group of highly conserved molecular chaperones critical for cellular stress responses. HSP70 proteins, including inducible (HSP70/HSPA1A) and constitutive (HSC70/HSPA8) isoforms, assist in protein folding, prevent aggregation of denatured proteins, and facilitate intracellular transport. Their expression increases under stress conditions such as heat, oxidative damage, or hypoxia, making them biomarkers of cellular stress.
In research, HSP70 antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate HSP70's roles in diseases. Elevated HSP70 levels are linked to cancer progression, neurodegenerative disorders (e.g., Alzheimer’s), and inflammatory conditions, while its downregulation may impair cellular repair mechanisms. These antibodies also help explore HSP70’s dual role in oncology—both promoting tumor survival and enhancing immunogenicity by presenting tumor antigens.
Commercially available HSP70 antibodies include monoclonal and polyclonal variants, often validated for cross-reactivity across species (human, mouse, rat). Challenges include distinguishing between isoforms and minimizing nonspecific binding. Clinically, HSP70 antibodies aid in diagnosing stress-related pathologies or evaluating therapeutic responses, particularly in cancer therapies targeting chaperone systems. Their utility underscores HSP70's importance in maintaining proteostasis and adapting to pathological challenges.
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