| WB | 1/500 - 1/2000 | Human,Monkey |
| IF | 咨询技术 | Human,Monkey |
| IHC | 咨询技术 | Human,Monkey |
| ICC | 技术咨询 | Human,Monkey |
| FCM | 咨询技术 | Human,Monkey |
| Elisa | 1/10000 | Human,Monkey |
| Aliases | TF2B; TFIIB; GTF2B |
| Entrez GeneID | 2959 |
| clone | 4C1 |
| WB Predicted band size | 34.8kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Monkey |
| Immunogen | Purified recombinant fragment of human GTF2B expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3-4条关于 **GTF2B(通用转录因子2B，即TFIIB)抗体** 的示例参考文献(基于领域知识概括，非真实文献，供参考格式)：
1. **文献名称**: "GTF2B-mediated recruitment of RNA polymerase II in transcriptional initiation complexes"
**作者**: Smith A, et al.
**摘要**: 研究通过染色质免疫沉淀(ChIP)和Western blot技术，利用GTF2B抗体证实了GTF2B在启动子区域与RNA聚合酶II及TFIID的共定位，揭示了其在转录起始复合物组装中的关键作用。
2. **文献名称**: "Dynamic interaction of GTF2B with chromatin during cell cycle progression"
**作者**: Lee C, et al.
**摘要**: 通过免疫荧光和流式细胞术结合GTF2B抗体，发现GTF2B在细胞周期不同阶段的核内分布变化，提示其参与细胞周期依赖性基因表达的调控。
3. **文献名称**: "Structural analysis of GTF2B in transcription pre-initiation complex assembly"
**作者**: Tanaka K, et al.
**摘要**: 利用抗GTF2B抗体进行免疫共沉淀(Co-IP)和冷冻电镜技术，解析了GTF2B与TFIIH、RNA聚合酶II的相互作用界面，阐明了其稳定转录前起始复合物的分子机制。
4. **文献名称**: "Aberrant GTF2B expression in cancer and its role in oncogenic transcription"
**作者**: Wang Y, et al.
**摘要**: 通过免疫组化(IHC)结合GTF2B抗体，发现多种癌症组织中GTF2B表达异常升高，体外实验表明其通过增强致癌基因(如MYC)的转录促进肿瘤进展。
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**注意**：以上为示例，实际文献需通过 **PubMed/Google Scholar** 等平台检索关键词(如“GTF2B antibody”或“TFIIB antibody”)获取。建议结合具体研究场景筛选文献。
The GTF2B antibody targets General Transcription Factor IIB (GTF2B, also known as TFIIB), a critical component of the RNA polymerase II-mediated transcription initiation complex. GTF2B plays a central role in assembling the pre-initiation complex (PIC) at promoter regions by bridging RNA polymerase II and transcription factor TFIID. It stabilizes the interaction between the TATA-box-binding protein (TBP) and promoter DNA, facilitates promoter melting, and ensures accurate transcription start site selection. Due to its essential function in basal transcription, GTF2B is ubiquitously expressed and highly conserved across eukaryotes.
Antibodies against GTF2B are widely used to study transcriptional regulation mechanisms, particularly in chromatin immunoprecipitation (ChIP), Western blotting (WB), and immunofluorescence (IF) assays. These tools help researchers investigate GTF2B’s interactions with other transcription factors, its recruitment dynamics during gene activation, and its role in diseases linked to transcriptional dysregulation, such as cancers or developmental disorders. Some studies also explore GTF2B’s involvement in viral infections, where pathogens hijack host transcription machinery.
Commercial GTF2B antibodies are typically raised in rabbits or mice using recombinant protein fragments or synthetic peptides. Validation often includes knockout cell lines or siRNA-mediated knockdown to confirm specificity. Researchers should verify cross-reactivity across species and applications, as performance may vary. Leading suppliers, such as Santa Cruz Biotechnology and Abcam, provide GTF2B antibodies with detailed protocols for experimental optimization.
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