| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DEFB129 |
| Uniprot No | Q9H1M3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-183aa |
| 氨基酸序列 | IGLRRCLMGLGRCRDHCNVDEKEIQKCKMKKCCVGPKVVKLIKNYLQYGTPNVLNEDVQEMLKPAKNSSAVIQRKHILSVLPQIKSTSFFANTNFVIIPNATPMNSATISTMTPGQITYTATSTKSNTKESRDSATASPPPAPPPPNILPTPSLELEEAEEQAAASFLEQKLISEEDLNSAVD |
| 预测分子量 | 21.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DEFB129重组蛋白的参考文献示例(注：以下内容为模拟生成，实际文献可能需要通过学术数据库查询确认)：
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1. **标题**: *"Recombinant DEFB129 Exhibits Antimicrobial Activity in the Human Reproductive Tract"*
**作者**: Schutte A., et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化了重组DEFB129蛋白，证实其在体外对多种泌尿生殖道病原体(如大肠杆菌和白色念珠菌)具有抗菌活性。结果表明DEFB129可能在男性生殖道黏膜免疫中起关键作用。
2. **标题**: *"Structural Characterization of Human Beta-Defensin 129 and Its Role in Sperm Motility"*
**作者**: Li Y., Zhou X., et al.
**摘要**: 通过核磁共振解析了DEFB129重组蛋白的三维结构，发现其通过静电相互作用与精子细胞膜结合，可能参与调节精子运动能力及保护生殖道免受感染。
3. **标题**: *"DEFB129 Gene Polymorphisms and Association with Vaginal Microbiota Imbalance"*
**作者**: Garcia-Rodriguez M., et al.
**摘要**: 研究探讨了DEFB129基因多态性与细菌性阴道病的关系，并利用重组蛋白实验证明特定突变体抗菌功能减弱，提示遗传变异可能影响宿主防御能力。
4. **标题**: *"Expression Optimization and Functional Screening of DEFB129 in Epithelial Cell Lines"*
**作者**: Kumar P., et al.
**摘要**: 优化了DEFB129在哺乳动物细胞中的重组表达体系，发现其能激活TLR4/NF-κB信号通路，提示除直接抗菌外，DEFB129可能参与免疫调节。
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建议通过 **PubMed** 或 **Web of Science** 以关键词“DEFB129 recombinant”“human beta-defensin 129”检索最新文献，或查阅《PLOS ONE》《Infection and Immunity》等期刊相关领域论文。
DEFB129 recombinant protein is derived from the human β-defensin 129 (DEFB129) gene, a member of the β-defensin family. β-defensins are small cationic antimicrobial peptides (AMPs) integral to innate immunity, primarily known for their broad-spectrum antimicrobial activity against bacteria, fungi, and viruses. These peptides are also implicated in immune modulation, wound healing, and reproductive system functions. DEFB129. specifically, is expressed in epithelial tissues, particularly in the male and female reproductive tracts, suggesting roles in mucosal defense and reproductive health. Studies suggest its involvement in sperm maturation, motility, and protection against pathogens in the urogenital environment.
The recombinant form of DEFB129 is produced using genetic engineering techniques, often via heterologous expression in bacterial (e.g., *E. coli*) or mammalian cell systems. This allows large-scale production of the purified, bioactive protein for research and therapeutic applications. Researchers utilize DEFB129 recombinant protein to study its structural properties, receptor interactions, and mechanisms underlying its antimicrobial and immunoregulatory functions. Its potential therapeutic applications include combating antibiotic-resistant infections, modulating inflammatory responses, or addressing fertility-related disorders.
Despite its promise, challenges remain in optimizing its stability, bioavailability, and tissue-specific targeting. Current research focuses on elucidating its precise physiological roles, post-translational modifications, and synergy with other host defense molecules. DEFB129’s dual role in immunity and reproduction makes it a unique candidate for interdisciplinary studies bridging immunology, microbiology, and reproductive biology.
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