| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | ZFH; Mi-2a; Mi2-ALPHA; CHD3 |
| Entrez GeneID | 1107 |
| clone | 2G4 |
| WB Predicted band size | 226kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Purified recombinant fragment of human CHD3 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是3篇涉及CHD3抗体的研究文献示例(文献信息为模拟示例，仅供参考)：
---
1. **文献名称**：*CHD3 loss disrupts chromatin organization at specific genomic loci and drives medulloblastoma progression*
**作者**：Smith J, et al. (2022)
**摘要**：研究利用CHD3特异性抗体进行ChIP-seq和免疫沉淀，揭示CHD3通过调控染色质结构抑制髓母细胞瘤关键致癌基因的异常激活，其缺失导致肿瘤恶化。
---
2. **文献名称**：*Germline mutations in CHD3 cause a neurodevelopmental disorder with impaired histone modification*
**作者**：Snijders Blok L, et al. (2020)
**摘要**：通过Western blot和免疫荧光实验(使用CHD3抗体)，发现CHD3基因突变患者细胞中染色质修饰异常，导致神经发育障碍，强调CHD3在表观遗传调控中的关键作用。
---
3. **文献名称**：*CHD3/NuRD complex restricts reprogramming by modulating TET1 activity*
**作者**：Wang H, et al. (2019)
**摘要**：研究采用CHD3抗体进行免疫共沉淀实验，证实CHD3作为NuRD复合体成员，通过抑制TET1介导的DNA去甲基化，维持体细胞多能性重编程的稳态。
---
4. **文献名称**：*Antibody-based profiling of CHD3 in lung adenocarcinoma identifies therapeutic vulnerabilities*
**作者**：Chen R, et al. (2021)
**摘要**：利用CHD3抗体进行组织芯片分析，发现CHD3低表达与肺癌患者生存率下降相关，并通过基因编辑验证其作为潜在治疗靶点的可行性。
---
**注**：以上文献信息为示例性质，实际引用时需核实真实文献来源及准确性。建议通过PubMed或Google Scholar以“CHD3 antibody”及“CHD3 function”等关键词检索最新研究。
CHD3 (Chromodomain Helicase DNA-binding protein 3) is a member of the CHD family of ATP-dependent chromatin remodeling proteins, which play critical roles in regulating gene expression by modifying chromatin structure. CHD3. along with CHD4 and CHD5. forms the core of the NuRD (Nucleosome Remodeling and Deacetylase) complex, which couples chromatin remodeling with histone deacetylase (HDAC) activity to repress transcription. This protein contains tandem chromodomains that recognize methylated histones, a helicase-like ATPase domain for nucleosome repositioning, and a PHD finger involved in protein interactions.
CHD3 is implicated in diverse cellular processes, including cell cycle regulation, DNA repair, and differentiation. Dysregulation of CHD3 has been linked to developmental disorders, such as Snijders Blok-Campeau syndrome (characterized by intellectual disability and speech impairment), and cancers, including glioblastoma and ovarian carcinoma. CHD3 antibodies are essential tools for studying its expression, localization, and interactions within the NuRD complex. They are widely used in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to explore its role in epigenetic regulation and disease mechanisms. Recent studies also highlight CHD3's potential as a therapeutic target, driving interest in antibodies for diagnostic and functional analyses.
×
