WB | 1/500 - 1/2000 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
FCM | 1/200 - 1/400 | Human,Mouse,Rat |
Elisa | 1/10000 | Human,Mouse,Rat |
Aliases | FABPI; I-FABP; MGC133132; FABP2 |
Entrez GeneID | 2169 |
clone | 9A9B7B3 |
WB Predicted band size | 15kDa |
Host/Isotype | Mouse IgG1 |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | Purified recombinant fragment of human FABP2 expressed in E. Coli. |
Formulation | Ascitic fluid containing 0.03% sodium azide. |
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以下是3篇关于FABP2抗体的参考文献概览(基于模拟数据生成,实际引用请核对原文):
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1. **文献名称**:*Fatty Acid-Binding Protein 2 (FABP2) as a Biomarker in Intestinal Inflammation*
**作者**:Smith A, et al.
**摘要**:研究通过FABP2抗体检测肠道炎症患者血清和活检样本中FABP2的表达水平,发现其与肠道通透性增加和炎症严重程度呈正相关,提示FABP2可作为炎症性肠病的潜在生物标志物。
2. **文献名称**:*Role of FABP2 in Lipid Metabolism: Insights from Knockout Mouse Models*
**作者**:Chen L, et al.
**摘要**:利用FABP2抗体进行免疫组化分析,发现FABP2缺失小鼠肠道上皮细胞的脂肪酸摄取能力显著降低,证实FABP2在脂质代谢中的关键作用,并为代谢综合征研究提供新靶点。
3. **文献名称**:*Development of a Monoclonal Antibody-Based ELISA for FABP2 Detection*
**作者**:Tanaka K, et al.
**摘要**:报道了一种高特异性抗FABP2单克隆抗体的开发,并基于此建立ELISA检测方法,验证其在结直肠癌患者粪便样本中的诊断灵敏度和特异性,为无创癌症筛查提供工具。
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如需具体文献,建议在PubMed或Web of Science中搜索关键词“FABP2 antibody”或结合研究领域(如肠道疾病、代谢研究)筛选。
Fatty Acid-Binding Protein 2 (FABP2), also known as intestinal FABP, is a 15 kDa cytoplasmic protein predominantly expressed in enterocytes of the small intestine. It plays a critical role in lipid metabolism by facilitating the uptake, intracellular transport, and processing of dietary long-chain fatty acids. FABP2 is encoded by the *FABP2* gene and is considered a biomarker for intestinal function and metabolic disorders due to its release into circulation during enterocyte damage.
FABP2 antibodies are immunological tools designed to detect and quantify FABP2 protein levels in research and clinical settings. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and enzyme-linked immunosorbent assays (ELISA) to study FABP2 expression patterns, subcellular localization, and regulatory mechanisms. In research, FABP2 antibodies help investigate associations between FABP2 polymorphisms or overexpression and metabolic conditions such as obesity, insulin resistance, and inflammatory bowel disease (IBD). Clinically, they aid in diagnosing intestinal ischemia, celiac disease, or other gut barrier dysfunction pathologies by detecting elevated FABP2 in blood or stool.
Recent studies also explore FABP2's role in gut-brain axis signaling and its potential as a therapeutic target. Validation of FABP2 antibodies ensures specificity for accurate experimental and diagnostic outcomes, supporting advancements in understanding intestinal health and metabolic disease mechanisms.
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