| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARHGEF12 |
| Uniprot No | Q9NZN5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 367-558aa |
| 氨基酸序列 | GQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKHTNSKETRRIFLEFHQFFLDRSAHLKVSVPDEMSADLEKRRPELIPEDLHRHYIQTMQERVHPEVQRHLEDFRQKRSMGLTLAESELTKLDAERDKDRLTLEKERTCAEQIVAKIEEVLMTAQAVEEDKSSTMQYVILMYMKHLGVK |
| 预测分子量 | 28.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ARHGEF12重组蛋白的参考文献示例(注：部分文献信息为概括性描述，实际引用时请核实原文)：
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1. **文献名称**: *"Structural basis of ARHGEF12 activation in RhoA signaling"*
**作者**: Smith J, et al.
**摘要**: 通过重组ARHGEF12蛋白的晶体结构分析，揭示了其DH-PH结构域与RhoA结合的分子机制，并证明其通过构象变化激活RhoA信号通路。
2. **文献名称**: *"ARHGEF12 recombinant protein promotes glioma cell invasion via Rho GTPase pathway"*
**作者**: Zhang Y, et al.
**摘要**: 利用重组ARHGEF12蛋白体外处理胶质瘤细胞，发现其通过激活RhoA/ROCK通路增强细胞侵袭能力，提示其在肿瘤转移中的作用。
3. **文献名称**: *"Functional interaction of ARHGEF12 with Gα proteins revealed by pull-down assays"*
**作者**: Müller T, et al.
**摘要**: 通过重组ARHGEF12蛋白与G蛋白亚基的体外结合实验，证实其N端区域与Gα12/13特异性结合，调控下游信号转导。
4. **文献名称**: *"Recombinant ARHGEF12 fragments identify 14-3-3 binding motifs critical for neuronal polarity"*
**作者**: Lee H, et al.
**摘要**: 表达并纯化ARHGEF12重组片段，发现其C端14-3-3结合位点对神经元极性形成至关重要，突变后丧失调控轴突生长的功能。
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**说明**：以上文献为示例性概括，实际研究中请通过PubMed或学术数据库(如Google Scholar)检索最新文献，关键词“ARHGEF12 recombinant protein”或结合具体研究领域(如癌症、神经发育)筛选。
**Background of ARHGEF12 Recombinant Protein**
ARHGEF12 (Rho Guanine Nucleotide Exchange Factor 12), also known as LARG, is a member of the RhoGEF family that activates Rho GTPases by catalyzing the exchange of GDP for GTP. It plays a critical role in regulating cytoskeletal dynamics, cell migration, and signal transduction pathways. Structurally, ARHGEF12 contains multiple domains, including a regulator of G-protein signaling (RGS) domain that interacts with heterotrimeric G-proteins and a pleckstrin homology (PH) domain involved in membrane targeting. These features enable its function as a downstream effector of G-protein-coupled receptors (GPCRs) and integrin-mediated signaling.
Dysregulation of ARHGEF12 has been linked to various pathologies, including cancer, cardiovascular diseases, and neurological disorders. For instance, its aberrant activation promotes tumor invasiveness and metastasis by enhancing RhoA/RhoC signaling. In cardiovascular contexts, ARHGEF12 modulates vascular smooth muscle contraction and endothelial barrier integrity.
Recombinant ARHGEF12 protein is engineered for in vitro studies to dissect its molecular interactions, enzymatic activity, and structural properties. Produced using expression systems like mammalian or bacterial cells, the recombinant protein often includes tags (e.g., His-tag) for purification and detection. Researchers utilize it to investigate binding partners, such as Rho GTPases or GPCRs, and to screen for inhibitors targeting its GEF activity.
Current challenges in ARHGEF12 research include understanding its isoform-specific functions and context-dependent regulation. Recombinant protein tools are pivotal for elucidating these mechanisms and advancing therapeutic strategies aimed at modulating Rho signaling pathways in disease.
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