Recombinant Human Ptk7 protein

中文名： 无活性的酪氨酸蛋白激酶7(Ptk7)重组蛋白
别名： Ptk7；CCK4；Inactive tyrosine-protein kinase 7

货号: PA2000-3834

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	Ptk7
Uniprot No	Q13308
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	31-704aa
氨基酸序列	AIVFIKQPSSQDALQGRRALLRCEVEAPGPVHVYWLLDGAPVQDTERRFAQGSSLSFAAVDRLQDSGTFQCVARDDVTGEEARSANASFNIKWIEAGPVVLKHPASEAEIQPQTQVTLRCHIDGHPRPTYQWFRDGTPLSDGQSNHTVSSKERNLTLRPAGPEHSGLYSCCAHSAFGQACSSQNFTLSIADESFARVVLAPQDVVVARYEEAMFHCQFSAQPPPSLQWLFEDETPITNRSRPPHLRRATVFANGSLLLTQVRPRNAGIYRCIGQGQRGPPIILEATLHLAEIEDMPLFEPRVFTAGSEERVTCLPPKGLPEPSVWWEHAGVRLPTHGRVYQKGHELVLANIAESDAGVYTCHAANLAGQRRQDVNITVATVPSWLKKPQDSQLEEGKPGYLDCLTQATPKPTVVWYRNQMLISEDSRFEVFKNGTLRINSVEVYDGTWYRCMSSTPAGSIEAQARVQVLEKLKFTPPPQPQQCMEFDKEATVPCSATGREKPTIKWERADGSSLPEWVTDNAGTLHFARVTRDDAGNYTCIASNGPQGQIRAHVQLTVAVFITFKVEPERTTVYQGHTALLQCEAQGDPKPLIQWKGKDRILDPTKLGPRMHIFQNGSLVIHDVAPEDSGRYTCIAGNSCNIKHTEAPLYVVDKPVPEESEGPGSPPPYKMIQT
预测分子量	76.6 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

1. **"PTK7 regulates Wnt signaling activity during early Xenopus development"**

*Author: Shnitsar I, Borchers A*

摘要：研究利用重组PTK7蛋白分析其在非洲爪蟾胚胎中的功能，发现PTK7通过调控非经典Wnt信号通路影响原肠胚形成和细胞极性建立。

2. **"Structural insights into the extracellular domain of PTK7 receptor"**

*Author: Jung JW, Lee SM, et al.*

摘要：通过表达并纯化人源PTK7重组胞外域蛋白，结合X射线晶体学解析其三维结构，揭示其与Wnt配体相互作用的关键结构域。

3. **"PTK7 as a potential therapeutic target in colorectal cancer"**

*Author: Zhang Y, Chen L, et al.*

摘要：利用重组PTK7蛋白进行体外功能实验，证明其通过激活Wnt/β-catenin信号促进结直肠癌细胞侵袭，提示其作为治疗靶点的潜力。

4. **"Recombinant PTK7-Fc融合蛋白抑制肿瘤血管生成"**

*Author: 王涛, 李芳, 等.*

摘要：构建PTK7胞外域-Fc融合蛋白，发现其通过竞争性结合VEGF抑制血管内皮细胞迁移及小鼠肿瘤模型中的血管生成。

背景信息

**Background of Ptk7 Recombinant Protein**

Ptk7 (Protein Tyrosine Kinase 7), also known as CCK4 (Colon Carcinoma Kinase 4), is a pseudokinase belonging to the receptor tyrosine kinase (RTK) family. Unlike typical kinases, Ptk7 lacks catalytic activity due to amino acid substitutions in its kinase domain. It plays critical roles in embryonic development, tissue homeostasis, and cell polarity by regulating signaling pathways such as Wnt/β-catenin and planar cell polarity (PCP). Ptk7 is essential for neural tube closure, angiogenesis, and directed cell migration, with dysregulation linked to developmental disorders and cancers.

The recombinant Ptk7 protein is engineered in vitro using expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, functional forms of the protein. This involves cloning the Ptk7 gene into a plasmid, transfecting host cells, and purifying the protein via affinity chromatography. Recombinant Ptk7 retains structural features, including extracellular immunoglobulin-like domains, a transmembrane region, and the intracellular pseudokinase domain, enabling studies on its molecular interactions.

Research applications of Ptk7 recombinant protein include elucidating its role in signaling crosstalk, particularly in modulating Wnt pathways, and exploring its potential as a therapeutic target. It is also used to generate antibodies, study protein-protein interactions, and model diseases like colorectal cancer and neural tube defects. The availability of recombinant Ptk7 accelerates functional studies and drug discovery, offering insights into its dual roles as a tumor suppressor or promoter, depending on cellular context.

Overall, Ptk7 recombinant protein serves as a vital tool for decoding its biological significance and developing targeted therapies for cancers and developmental disorders.