| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EBNA2 |
| Uniprot No | Q69022 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 247-454aa |
| 氨基酸序列 | MPTYYLALHGGQSYNLIVDTDMSGNPSLSVIPTNPYQEQLSNNPLIQLQI VVGENTGAPAPPQPPPPPPPPPPPERRDAWTQEPLPLDMNPLGSDASQGP LASSIRMLCMAQYLLRNARGQQGLLRPLGPQTRSQVTLERQPVHNPRQEA PIILLQSPAPPRFTPVPMVALGHTLQPTPPPRPTLPQPRIPLIIPPRHTN QPATTPPTAPQRLTLGHQLSLPPHPPPHQSTPHCSSDSTGLPPPPTSYSI PSMTLSPEPLPPPAAPAHPLPGVIYDQQALPPTPGPPWWPPVRDPTPTTQ TPPTNTKQGPDQGQGRGRWRGRGRSKGRGRMHKLPEPRRPGPDTSSPSMP QLSPVVSLHQGQGPENSPTPGPSTAGPVCRVTPSATPDISPIHEPESSDS EEPPFLFPSDWYPPTLEPAELDESWEGIFETTESHSSDEENVGGPSKRPR TSTQ |
| 预测分子量 | 48,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于EBNA2重组蛋白的相关文献概览：
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1. **文献名称**: *"Epstein-Barr virus nuclear antigen 2 interactions with host cellular proteins"*
**作者**: Cohen, J.I., & Kieff, E.
**摘要**: 研究EBNA2重组蛋白与宿主细胞蛋白(如RBP-Jκ)的相互作用，揭示其通过劫持Notch信号通路激活病毒潜伏期基因表达的机制。
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2. **文献名称**: *"Structural basis of DNA recognition by the EBNA2 protein for Epstein-Barr virus latency establishment"*
**作者**: Peng, C.W., et al.
**摘要**: 通过X射线晶体学解析EBNA2重组蛋白的DNA结合域结构，阐明其如何特异性识别病毒基因组中的调控序列以启动潜伏感染。
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3. **文献名称**: *"Recombinant EBNA2 protein induces B-cell immortalization in vitro"*
**作者**: Hammerschmidt, W., & Sugden, B.
**摘要**: 利用重组EBNA2蛋白体外实验证明其单独表达足以驱动B细胞永生化，验证其在EB病毒致癌性中的核心作用。
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4. **文献名称**: *"EBNA2 post-translational modifications regulate its transcriptional activity"*
**作者**: Zhou, H., et al.
**摘要**: 分析重组EBNA2蛋白的磷酸化和乙酰化修饰，发现这些修饰通过影响其与转录共激活因子的结合来调控靶基因表达水平。
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**注**：以上文献为示例性质，实际文献需通过PubMed、Web of Science等平台检索确认。
EBNA2 (Epstein-Barr nuclear antigen 2) is a critical regulatory protein encoded by the Epstein-Barr virus (EBV), a gammaherpesvirus associated with several human malignancies, including Burkitt’s lymphoma, Hodgkin’s lymphoma, and nasopharyngeal carcinoma. EBNA2 is essential for EBV-mediated B-cell immortalization and viral latency establishment. It functions as a transcriptional coactivator, binding to cellular DNA through interactions with host transcription factors like RBP-Jκ (recombination signal-binding protein for immunoglobulin kappa J region) and PU.1. thereby modulating the expression of viral and host genes to promote cell proliferation and survival.
Recombinant EBNA2 protein is produced using heterologous expression systems (e.g., bacterial, insect, or mammalian cells) for functional and structural studies. Its modular structure includes an N-terminal transactivation domain and a C-terminal domain involved in protein-protein interactions. Researchers utilize recombinant EBNA2 to investigate its role in viral oncogenesis, particularly its ability to hijack host signaling pathways (e.g., Notch signaling via RBP-Jκ binding) and alter epigenetic regulation. Studies also focus on its interaction with viral promoters (e.g., Cp and LMP1/2 promoters) to sustain latent infection.
Purified EBNA2 recombinant protein serves as a tool for in vitro assays, antibody production, and drug screening targeting EBV-associated diseases. Understanding its molecular mechanisms aids in developing therapeutic strategies to disrupt EBNA2-host interactions, potentially inhibiting viral persistence and oncogenesis. Despite progress, challenges remain in resolving its full structure and context-dependent regulatory networks, underscoring the need for continued research using recombinant protein models.
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