| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GIGYF2 |
| Uniprot No | Q6Y7W6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 510-1030aa |
| 氨基酸序列 | ERLASKLQEHRAKGVSIPLMHEAMQKWYYKDPQGEIQGPFNNQEMAEWFQAGYFTMSLLVKRACDESFQPLGDIMKMWGRVPFSPGPAPPPHMGELDQERLTRQQELTALYQMQHLQYQQFLIQQQYAQVLAQQQKAALSSQQQQQLALLLQQFQTLKMRISDQNIIPSVTRSVSVPDTGSIWELQPTASQPTVWEGGSVWDLPLDTTTPGPALEQLQQLEKAKAAKLEQERREAEMRAKREEEERKRQEELRRQQEEILRRQQEEERKRREEEELARRKQEEALRRQREQEIALRRQREEEERQQQEEALRRLEERRREEEERRKQEELLRKQEEEAAKWAREEEEAQRRLEENRLRMEEEAARLRHEEEERKRKELEVQRQKELMRQRQQQQEALRRLQQQQQQQQLAQMKLPSSSTWGQQSNTTACQSQATLSLAEIQKLEEERERQLREEQRRQQRELMKALQQQQQQQQQKLSGWGNVSKPSGTTKSLLEIQQEEARQMQKQQQQQQQHQQPNRAR |
| 预测分子量 | 69.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4篇关于GIGYF2重组蛋白的参考文献及其摘要内容的简要概括：
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1. **文献名称**: *GIGYF2 and 4EHP inhibit translation initiation of defective mRNAs and assist ribosome-associated protein quality control*
**作者**: Varga et al.
**摘要**: 研究揭示了GIGYF2通过与翻译抑制因子4EHP结合，参与核糖体相关质量控制(RQC)机制，识别停滞的翻译复合物，并调控缺陷mRNA的降解，维持蛋白质稳态。
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2. **文献名称**: *Structural basis for the interaction between the IGHMBP2 protein and GIGYF2 in the regulation of mTOR signaling*
**作者**: Trempe et al.
**摘要**: 通过X射线晶体学解析GIGYF2与IGHMBP2的复合物结构，揭示了二者在调控mTOR信号通路中的相互作用机制，为神经退行性疾病相关突变的功能研究提供结构基础。
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3. **文献名称**: *Parkinson’s disease-linked mutations in GIGYF2 disrupt selective autophagy pathways*
**作者**: Deng et al.
**摘要**: 报道GIGYF2突变通过干扰自噬受体OPTN的招募，损害错误折叠蛋白的清除，从而促进帕金森病中α-突触核蛋白的积累，揭示了其与神经退行性疾病的直接关联。
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4. **文献名称**: *Two homologous proteins, GIGYF1 and GIGYF2. define a novel family of signaling adaptors for receptor tyrosine kinases*
**作者**: Giovannone et al.
**摘要**: 首次克隆并表征GIGYF2重组蛋白，发现其与胰岛素样生长因子1受体(IGF-1R)等酪氨酸激酶受体结合，参与生长因子信号传导调控，提示其在细胞增殖和代谢中的潜在作用。
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以上文献涵盖GIGYF2的分子机制、结构解析及疾病关联研究，均为领域内代表性工作。
**Background of GIGYF2 Recombinant Protein**
GIGYF2 (Grb10-Interacting GYF Protein 2) is a multifunctional protein encoded by the *GIGYF2* gene, located on human chromosome 2q37.1. It belongs to the GYF domain-containing protein family, characterized by a conserved glycine-tyrosine-phenylalanine (GYF) motif that mediates protein-protein interactions. GIGYF2 is primarily involved in regulating cellular signaling pathways, including insulin/insulin-like growth factor (IGF) signaling, endosomal trafficking, and translational control. It interacts with Grb10 (Growth Factor Receptor-Bound Protein 10), an adaptor protein linked to receptor tyrosine kinase signaling, and modulates downstream targets such as mTOR and 4E-BP1. influencing cell growth, proliferation, and metabolism.
GIGYF2 has gained attention due to its association with neurodegenerative disorders. Specific mutations in *GIGYF2* have been implicated in familial Parkinson’s disease (PD), though their pathogenicity remains debated. Studies suggest that GIGYF2 dysfunction may impair vesicle trafficking and protein homeostasis, contributing to dopaminergic neuron degeneration. Additionally, GIGYF2 interacts with components of the mRNA translation machinery, hinting at roles in stress granule formation and RNA metabolism under cellular stress.
Recombinant GIGYF2 protein is engineered for in vitro and in vivo studies to dissect its molecular mechanisms. Produced via bacterial (e.g., *E. coli*) or mammalian expression systems, it is often tagged (e.g., His, GST) for purification and detection. Researchers utilize this protein to investigate its structural domains, binding partners, and functional alterations caused by PD-linked mutations. It also serves as a tool for screening therapeutic compounds targeting GIGYF2-related pathways.
Despite progress, many aspects of GIGYF2 biology, including its tissue-specific roles and cross-talk with other PD-associated proteins (e.g., LRRK2. α-synuclein), remain under exploration. Its dual roles in signaling and neurodegeneration make it a compelling target for interdisciplinary research.
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