| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | HDT2 |
| Uniprot No | Q56WH4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-306aa |
| 氨基酸序列 | MEFWGVAVTPKNATKVTPEEDSLVHISQASLDCTVKSGESVVLSVTVGGA KLVIGTLSQDKFPQISFDLVFDKEFELSHSGTKANVHFIGYKSPNIEQDD FTSSDDEDVPEAVPAPAPTAVTANGNAGAAVVKADTKPKAKPAEVKPAEE KPESDEEDESDDEDESEEDDDSEKGMDVDEDDSDDDEEEDSEDEEEEETP KKPEPINKKRPNESVSKTPVSGKKAKPAAAPASTPQKTEEKKKGGHTATP HPAKKGGKSPVNANQSPKSGGQSSGGNNNKKPFNSGKQFGGSNNKGSNKG KGKGRA |
| 预测分子量 | 34 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HDT2重组蛋白的3篇假设性参考文献示例(基于常见研究方向推测，实际文献需通过数据库检索确认)：
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1. **文献名称**: *"Recombinant HDT2 Protein Expression and Its Role in Histone Deacetylation"*
**作者**: Zhang L, et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化HDT2重组蛋白，验证其组蛋白去乙酰化酶活性，并发现HDT2通过调控特定组蛋白修饰影响植物(拟南芥)的耐旱性。
2. **文献名称**: *"HDT2 Recombinant Protein Inhibits Cancer Cell Proliferation via Epigenetic Modulation"*
**作者**: Kim S, et al.
**摘要**: 通过体外实验证明，重组HDT2蛋白可特异性结合肿瘤细胞染色质，降低关键癌基因启动子区域的乙酰化水平，从而抑制肝癌细胞增殖。
3. **文献名称**: *"Structural and Functional Characterization of the HDT2 Deacetylase Domain"*
**作者**: Wang Y, et al.
**摘要**: 利用重组HDT2蛋白的晶体结构解析，揭示其催化核心的锌离子结合位点，并发现小分子抑制剂可靶向该区域，为开发表观遗传药物提供依据。
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**注**：以上为模拟参考文献，实际研究中请通过PubMed、Web of Science或Google Scholar检索关键词 **"HDT2 recombinant protein"** 或 **"HDT2 histone deacetylase"** 获取真实文献。若研究领域为植物学，可补充检索 **"Arabidopsis HDT2"**。
The HDT2 recombinant protein is derived from the Histone Deacetylase 2 (HD2) family, a class of enzymes involved in epigenetic regulation through post-translational modification of histones. Specifically, HDT2 belongs to the plant-specific HD2 subfamily, initially identified in *Arabidopsis thaliana*, where it plays a critical role in chromatin remodeling and gene silencing. Unlike classical HDACs (Histone Deacetylases), HD2 proteins exhibit unique structural features, such as a conserved N-terminal nuclear localization signal and a C-terminal catalytic domain, enabling site-specific deacetylation of histone H3 and H4 lysine residues. This activity modulates chromatin compaction, influencing transcriptional repression, heterochromatin formation, and developmental processes.
Recombinant HDT2 is engineered using molecular cloning techniques, where the *HDT2* gene is inserted into expression vectors (e.g., *E. coli* or yeast systems) to produce purified protein for functional studies. Its recombinant form retains enzymatic activity, allowing researchers to study its role in vitro or in heterologous systems. Applications include investigating epigenetic mechanisms in plant stress responses, seed development, and floral transition. Additionally, HDT2’s interaction with other chromatin modifiers (e.g., DNA methyltransferases) and non-coding RNAs has spurred interest in its potential for agricultural biotechnology, such as enhancing crop resilience. Studies also explore its divergence from animal HDACs, offering insights into evolutionary adaptations of epigenetic regulation. Overall, HDT2 recombinant protein serves as a vital tool for dissecting plant epigenetics and developing strategies to manipulate gene expression in biotechnological contexts.
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