| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | ssaA1 |
| Uniprot No | Q5HCY4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 27-255aa |
| 氨基酸序列 | AEQNNNGYNSNDAQSYSYTYTIDAQGNYHYTWTGNWNPSQLTQNNTYYYNNYNTYSYNNASYNNYYNHSYQYNNYTNNSQTATNNYYTGGSGASYSTTSNNVHVTTTAAPSSNGRSISNGYASGSNLYTSGQCTYYVFDRVGGKIGSTWGNASNWANAAASSGYTVNNTPKVGAIMQTTQGYYGHVAYVEGVNSNGSVRVSEMNYGHGAGVVTSRTISANQAGSYNFIH |
| 预测分子量 | 27.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇与ssaA1重组蛋白相关的虚构参考文献示例(实际文献需通过学术数据库核实)：
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1. **文献名称**: *"Cloning and functional characterization of Salmonella Typhimurium ssaA1 gene encoding a T3SS effector protein"*
**作者**: Müller, J. et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化重组ssaA1蛋白，证实其通过沙门氏菌III型分泌系统(T3SS)调控宿主细胞信号通路，促进细菌胞内存活。
2. **文献名称**: *"Recombinant ssaA1 as a potential subunit vaccine against Salmonella infection"*
**作者**: Zhang, Y. et al.
**摘要**: 通过原核系统表达ssaA1重组蛋白，动物实验表明其能诱导小鼠产生高水平IgG抗体及Th1型免疫应答，显著降低沙门氏菌攻毒后的脏器载菌量。
3. **文献名称**: *"Structural analysis of SsaA1 reveals key motifs for host-pathogen interaction"*
**作者**: García-Ramírez, L. et al.
**摘要**: 利用X射线晶体学解析ssaA1蛋白三维结构，发现其N端结构域与宿主细胞膜磷脂特异性结合，为开发靶向抑制剂奠定基础。
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如需真实文献，建议通过PubMed或Web of Science检索关键词：**ssaA1 recombinant protein, Salmonella pathogenicity island 2 (SPI-2), type III secretion system**。部分研究可能聚焦于ssaA1在毒力或宿主互作中的机制。
The SsaA1 recombinant protein is derived from the SsaA1 gene, which encodes a key component of the Type III Secretion System (T3SS) in *Salmonella enterica* serovars, particularly those associated with systemic infections. This secretion system, located within Salmonella Pathogenicity Island 2 (SPI-2), is critical for bacterial survival and replication within host cells, enabling the pathogen to evade immune responses and establish persistent infections. The SsaA1 protein functions as a structural and regulatory element of the SPI-2 T3SS apparatus, facilitating the translocation of effector proteins into host cells to manipulate cellular processes, such as vesicle trafficking and immune signaling.
Recombinant SsaA1 is typically produced using heterologous expression systems, such as *Escherichia coli*, through cloning and overexpression of the ssaA1 gene. Purification methods often involve affinity chromatography (e.g., His-tag systems) followed by refolding or buffer exchange to ensure proper protein conformation. Its production enables detailed study of SPI-2 T3SS assembly, host-pathogen interactions, and virulence mechanisms. Researchers utilize SsaA1 recombinant protein to investigate immune responses, including antibody production and T-cell activation, as well as to develop diagnostic tools or subunit vaccines targeting *Salmonella*. Additionally, it serves as a molecular tool to dissect the role of T3SS in intracellular bacterial survival, offering insights into broader antimicrobial strategies. Studies on SsaA1 contribute to understanding Gram-negative bacterial pathogenesis and the design of therapeutics against antibiotic-resistant strains.
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