| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Fbxw10 |
| Uniprot No | Q5XX13 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1052aa |
| 氨基酸序列 | MENLESRLKNAPYFRCEKGTDSIPLCRKCETCVLAWKIFSTKEWFCRINDISQRRFLVGILKQLNSLYLLHYFQNILQTTQGKDFIYNRSRINLSKKEGKVVKSSLNQMLDKTVEQKMKEILYWFANSTQWTKANYTLLLLQMCNPKLLLTAANVIRVLFLREENNISGLNQDITDVCFSPEKDHSSKSATSQVYWTAKTQHTSLPLSKAPENEHLLGAASNPEEPWRNSLRCISEMNRLFSGKGDITKPGYDPCNLLVDLDDIRDLSSGFSKYRDFIRYLPIHLSKYILRMLDRHTLNKCASVSQHWAAMAQQVKMDLSAHGFIQNQITFLQGSYTRGIDPNYANKVSIPVPKMVDDGKSMRVKHPKWKLRTKNEYNLWTAYQNEETQQVLIEERNVFCGTYNVRILSDTWDQNRVIHYSGGDLIAVSSNRKIHLLDIIQVKAIPVEFRGHAGSVRALFLCEEENFLLSGSYDLSIRYWDLKSGVCTRIFGGHQGTITCMDLCKNRLVSGGRDCQVKVWDVDTGKCLKTFRHKDPILATRINDTYIVSSCERGLVKVWHIAMAQLVKTLSGHEGAVKCLFFDQWHLLSGSTDGLVMAWSMVGKYERCLMAFKHPKEVLDVSLLFLRVISACADGKIRIYNFLNGNCMKVLKANGRGDPVLSFFIQGNRMVVNTESNVLMFQFEHIKWQYAVEKTKQKKNKEKEEEKEENSLMEILSKCNIQVHSPRESVSSKQTVIQELLPGKPPKSRVLLKPAKFSSAVLIEELQSQGKSKSPRRDADDVEKAQKQGQLETPGKLPSHPKKKSWKIPMSPDQFLLTVSALQHAHNSGEFAYPCRPQTEITDVWGPSISYPRKVLNFKGKSIQRAVDRLRLSNPPIDVKRTSIPLEIQKLQPNLKISLHSPRVQSTIPQPMIIRSRFSGSLKGGDQVTSSIERAVCSTGPLTSMQVIKPNRMLAPQVGTATLSLKKERPRIYTALDPFRVNTEFVLLTVKEEKEHQEAKMKEYQARESTGVVDPGKVSKAAWIRKIKGLPIDNFTKQGKTAAPELGQNVFI |
| 预测分子量 | 119,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Fbxw10重组蛋白的3篇示例文献(内容为模拟，供参考)：
1. **文献名称**：*"FBXW10 regulates mitochondrial dynamics via ubiquitination of Mfn2 in mammalian cells"*
**作者**：Kim, J. et al.
**摘要**：本研究通过重组表达FBXW10蛋白，发现其作为E3泛素连接酶复合物的底物识别亚基，靶向线粒体融合蛋白Mfn2并介导其泛素化降解，从而调控线粒体分裂过程。
2. **文献名称**：*"Recombinant FBXW10 inhibits cell proliferation by promoting Cyclin E degradation in hepatocellular carcinoma"*
**作者**：Wang, L. et al.
**摘要**：作者利用昆虫表达系统制备重组FBXW10蛋白，证明其通过结合并泛素化细胞周期蛋白Cyclin E，诱导蛋白酶体依赖性降解，抑制肝癌细胞增殖。
3. **文献名称**：*"Structural characterization of FBXW10-F-box domain and its interaction with Skp1"*
**作者**：Garcia-Rodriguez, C. et al.
**摘要**：通过重组表达FBXW10的F-box结构域，结合X射线晶体学分析其与Skp1蛋白的相互作用界面，揭示了FBXW10参与SCF泛素连接酶复合物组装的分子机制。
---
**注**：以上文献为示例，实际研究中Fbxw10的相关文献较少，建议结合**UniProt(ID: Q5TFE4)**或**NCBI Gene(Gene ID: 261734)**数据库获取基础信息，并通过PubMed以“FBXW10 AND recombinant”为关键词检索最新研究。
**Background of FBXW10 Recombinant Protein**
FBXW10 (F-box/WD repeat-containing protein 10) is a member of the F-box protein family, which functions as a substrate-recognition component of the SCF (SKP1-CUL1-F-box) E3 ubiquitin ligase complex. This complex plays a central role in the ubiquitin-proteasome system (UPS), regulating protein degradation and maintaining cellular homeostasis. The F-box domain of FBXW10 mediates interactions with SKP1. linking it to the core SCF machinery, while its WD40 repeats are thought to facilitate substrate binding.
FBXW10 is less characterized compared to other F-box proteins like FBXW7. a well-studied tumor suppressor. Current research suggests FBXW10 may participate in cell cycle regulation, signal transduction, and apoptosis by targeting specific proteins for ubiquitination and subsequent proteasomal degradation. Its expression appears tissue-specific, with higher levels detected in testis, implying potential roles in germ cell development or reproductive biology. However, its precise biological functions and physiological substrates remain under investigation.
Recombinant FBXW10 protein is engineered for *in vitro* studies to elucidate its molecular interactions, enzymatic activity, and regulatory mechanisms. Produced using heterologous expression systems (e.g., *E. coli* or mammalian cells), the recombinant form often includes affinity tags (e.g., GST, His-tag) for purification and detection. Such tools enable researchers to analyze FBXW10’s binding partners, assess its role in ubiquitination cascades, and explore its relevance to diseases, including cancer or infertility.
Despite its potential significance, FBXW10 research is nascent, with gaps in understanding its exact regulatory networks and pathological implications. Ongoing studies aim to map its interactome, validate putative substrates, and define its contribution to cellular pathways, paving the way for therapeutic targeting in associated disorders.
×
