| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RHOT1 |
| Uniprot No | Q8IXI2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-681aa |
| 氨基酸序列 | MKKDVRILLVGEPRVGKTSLIMSLVSEEFPEEVPPRAEEITIPADVTPERVPTHIVDYSEAEQSDEQLHQEISQANVICIVYAVNNKHSIDKVTSRWIPLINERTDKDSRLPLILVGNKSDLVEYSSMETILPIMNQYTEIETCVECSAKNLKNISELFYYAQKAVLHPTGPLYCPEEKEMKPACIKALTRIFKISDQDNDGTLNDAELNFFQRICFNTPLAPQALEDVKNVVRKHISDGVADSGLTLKGFLFLHTLFIQRGRHETTWTVLRRFGYDDDLDLTPEYLFPLLKIPPDCTTELNHHAYLFLQSTFDKHDLDRDCALSPDELKDLFKVFPYIPWGPDVNNTVCTNERGWITYQGFLSQWTLTTYLDVQRCLEYLGYLGYSILTEQESQASAVTVTRDKKIDLQKKQTQRNVFRCNVIGVKNCGKSGVLQALLGRNLMRQKKIREDHKSYYAINTVYVYGQEKYLLLHDISESEFLTEAEIICDVVCLVYDVSNPKSFEYCARIFKQHFMDSRIPCLIVAAKSDLHEVKQEYSISPTDFCRKHKMPPPQAFTCNTADAPSKDIFVKLTTMAMYPHVTQADLKSSTFWLRASFGATVFAVLGFAMYKALLKQR |
| 预测分子量 | 70,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RHOT1(Miro1)重组蛋白的参考文献示例，内容基于典型研究方向整理：
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1. **标题**: *"Structural characterization of the Miro GTPase domain using recombinant protein expression"*
**作者**: Müller, T. et al. (2021)
**摘要**: 通过重组表达纯化人源Miro1的GTP酶结构域，结合X射线晶体学解析其三维结构，揭示了其与钙离子结合的构象变化，为线粒体运动调控机制提供结构基础。
2. **标题**: *"Recombinant RHOT1 restores mitochondrial motility in axonal transport-deficient neurons"*
**作者**: López-Doménech, G. et al. (2018)
**摘要**: 利用重组RHOT1蛋白在神经元模型中恢复线粒体轴突运输功能，证明其通过与驱动蛋白KIF5的相互作用介导线粒体前向运动，并探讨其在神经退行性疾病中的潜在应用。
3. **标题**: *"Calcium-sensitive regulation of Miro1 via recombinant protein reconstitution in vitro"*
**作者**: Wang, X. et al. (2020)
**摘要**: 通过体外重组蛋白实验，证明Miro1的钙离子依赖性构象变化可解除其与微管的锚定，从而调控线粒体在细胞内的停滞机制。
4. **标题**: *"High-yield purification of recombinant Miro1 and its role in Parkinson’s disease-linked pathways"*
**作者**: Birsa, N. et al. (2019)
**摘要**: 开发了一种高效重组Miro1蛋白纯化方法，并发现其在帕金森病相关蛋白PINK1/Parkin介导的线粒体自噬通路中起关键作用。
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**备注**：上述文献为示例，实际引用时需核实具体来源及发表详情。建议通过PubMed或Google Scholar以关键词“RHOT1 recombinant”“Miro1 purification”“Miro1 mitochondrial transport”检索最新研究。
RHOT1 (Ras Homolog Family Member T1), also known as Miro1. is a mitochondrial Rho GTPase that plays a critical role in regulating mitochondrial dynamics and intracellular transport. As a key component of the mitochondrial outer membrane, RHOT1 facilitates the movement of mitochondria along microtubules by interacting with adaptor proteins like TRAK1/2 and the kinesin motor complex. This process ensures proper mitochondrial distribution to meet localized energy demands and support cellular functions, particularly in polarized cells such as neurons. Structurally, RHOT1 contains two GTPase domains flanking EF-hand calcium-binding motifs, enabling it to act as a calcium-sensitive regulator of mitochondrial motility.
Recombinant RHOT1 protein is commonly engineered for in vitro studies to dissect its molecular interactions, GTPase activity, and calcium-dependent conformational changes. Researchers utilize expression systems like E. coli or mammalian cells to produce purified RHOT1. often with affinity tags (e.g., His-tag) for simplified isolation. This recombinant tool has become essential for investigating mitochondrial dysfunction linked to neurodegenerative diseases (e.g., Parkinson's and Alzheimer's), cancer metastasis, and metabolic disorders. Recent studies also employ RHOT1 variants to explore its role in mitophagy regulation and apoptosis. However, challenges persist in maintaining the protein's native conformation due to its transmembrane domains and post-translational modifications, driving ongoing optimization of recombinant expression strategies.
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