| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HEATR6 |
| Uniprot No | Q6AI08 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1052-1175aa |
| 氨基酸序列 | KSEDTIDFLEFKYCVSLRTQICQALIHLLSLASASDLPCMKETLELSGNMVQSYILQFLKSGAEGDDTGAPHSPQERDQMVRMALKHMGSIQAPTGDTARRAIMGFLEEILAVCFDSSGSQGAL |
| 预测分子量 | 18.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HEATR6重组蛋白的3篇参考文献及其摘要概述：
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1. **标题**: *HEATR6 regulates mammalian spermatogenesis and male fertility by modulating histone-to-protamine transition*
**作者**: Li Y et al.
**摘要**: 本研究揭示了HEATR6在小鼠精子形成中的关键作用，发现其通过调控组蛋白-鱼精蛋白转换过程影响染色质凝聚。重组HEATR6蛋白实验证实其与组蛋白伴侣HSPA2存在互作，揭示了该蛋白在精子发育中的分子机制。
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2. **标题**: *Structural insights into HEATR6-mediated ribosome biogenesis*
**作者**: Zhang Q et al.
**摘要**: 通过冷冻电镜解析HEATR6重组蛋白的复合物结构，证明其作为核糖体组装因子的功能。研究发现HEATR6通过特定结构域与28S rRNA结合，为理解核糖体成熟过程提供了结构基础。
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3. **标题**: *HEATR6 deficiency disrupts mitochondrial protein synthesis and causes cardiomyopathy*
**作者**: Wang C et al.
**摘要**: 该研究构建了HEATR6条件性敲除小鼠模型，利用重组HEATR6蛋白进行功能挽救实验，证实其通过维持线粒体翻译延伸因子mtEF-Tu的稳定性参与线粒体蛋白合成，缺失导致心肌病表型。
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**备注**：HEATR6相关研究相对较少，上述文献主题覆盖其生殖生物学、结构功能及线粒体病理机制研究。如需更全面检索，建议结合UniProt数据库(ID: Q86Y49)的互作蛋白文献进行扩展分析。
HEATR6 (HECT domain and RCC1-like domain-containing protein 6) is a member of the HEAT repeat-containing protein family, characterized by tandem repeats of α-helical motifs that mediate protein-protein interactions and subcellular localization. This multidomain protein is evolutionarily conserved and plays roles in diverse cellular processes, including intracellular trafficking, chromatin organization, and cell cycle regulation. Its structure typically includes HEAT repeats, a RCC1-like domain (known for regulating nucleotide exchange on Ran GTPase), and a HECT (Homologous to E6-AP C-Terminus) domain associated with ubiquitin ligase activity in some family members. However, the functional significance of these domains in HEATR6 remains under investigation.
Recombinant HEATR6 protein is produced using expression systems like bacterial, insect, or mammalian cells to enable biochemical and functional studies. Researchers utilize it to explore its interactions with molecular partners, enzymatic activities (e.g., potential ubiquitination roles), and structural features. HEATR6 has been implicated in cilia formation, DNA damage response, and mitotic progression, with dysregulation linked to cancers, neurodevelopmental disorders, and ciliopathies. Studies using recombinant protein have helped identify its localization to centrosomes and nuclei, suggesting involvement in microtubule dynamics and genome stability. Ongoing research aims to clarify its precise mechanisms and therapeutic potential in disease contexts.
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