| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ERVK-6 |
| Uniprot No | Q69384 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 90-632aa |
| 氨基酸序列 | LPMPAGAAAANYTYWAYVPFPPLIRAVTWMDNPTEVYVNDSVWVPGPIDDRCPAKPEEEGMMINISIGYHYPPICLGRAPGCLMPAVQNWLVEVPTVSPICRFTYHMVSGMSLRPRVNYLQDFSYQRSLKFRPKGKPCPKEIPKESKNTEVLVWEECVANSAVILQNNEFGTIIDWAPRGQFYHNCSGQTQSCPSAQVSPAVDSDLTESLDKHKHKKLQSFYPWEWGEKGISTPRPKIVSPVSGPEHPELWRLTVASHHIRIWSGNQTLETRDRKPFYTIDLNSSLTVPLQSCVKPPYMLVVGNIVIKPDSQTITCENCRLLTCIDSTFNWQHRILLVRAREGVWIPVSMDRPWEASPSVHILTEVLKGVLNRSKRFIFTLIAVIMGLIAVTATAAVAGVALHSSVQSVNFVNDWQKNSTRLWNSQSSIDQKLANQINDLRQTVIWMGDRLMSLEHRFQLQCDWNTSDFCITPQIYNESEHHWDMVRRHLQGREDNLTLDISKLKEQIFEASKAHLNLVPGTEAIAGVADGLANLNPVTWVKT |
| 预测分子量 | 64 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ERVK-6重组蛋白的模拟参考文献示例(请注意，部分信息为基于领域研究的模拟内容，非真实文献)：
---
1. **《HERV-K (HML-6) Envelope Recombinant Protein Induces Autoantibody Response in Breast Cancer》**
*作者：Smith A et al.*
摘要：研究通过重组表达HERV-K6包膜蛋白，发现其在乳腺癌患者血清中可诱导特异性自身抗体，提示其可能作为癌症生物标志物或免疫治疗靶点。
2. **《Structural and Functional Analysis of ERVK-6 Recombinant Envelope Glycoprotein》**
*作者：Li Y et al.*
摘要：利用X射线晶体学解析了重组ERVK-6包膜蛋白的胞外域结构，揭示了其与宿主细胞受体相互作用的潜在机制，为开发靶向抑制剂提供依据。
3. **《Recombinant ERVK-6 Protein Activates Innate Immune Response via TLR4 Pathway》**
*作者：Garcia R et al.*
摘要：实验证明，重组ERVK-6蛋白可通过激活TLR4信号通路触发巨噬细胞炎症反应，提示内源性逆转录病毒蛋白在自身免疫疾病中的潜在作用。
4. **《Expression and Purification of Recombinant ERVK-6 Gag Protein for Retroviral Assembly Studies》**
*作者：Chen L et al.*
摘要：优化了ERVK-6 Gag重组蛋白的原核表达系统，并验证其在体外自组装病毒样颗粒(VLPs)的能力，为研究逆转录病毒组装机制提供工具。
---
**注意**：以上文献信息为模拟生成，实际研究中请通过PubMed、Web of Science等平台检索真实文献。若需具体文章，可提供更多关键词进一步筛选。
ERVK-6 recombinant protein is derived from the human endogenous retrovirus K (HERV-K) family, specifically the envelope (Env) glycoprotein of the HERV-K(HML-6) subgroup. HERVs are remnants of ancient retroviral infections integrated into the human genome, constituting ~8% of human DNA. While most HERVs are silenced or degraded, some retain functional open reading frames, with HERV-K being one of the most recently active and biologically relevant subgroups. The HML-6 Env protein, encoded by the *env* gene, is implicated in physiological and pathological processes, including placental development, immune modulation, and cellular fusion.
ERVK-6 recombinant protein is engineered through molecular cloning, typically expressed in mammalian or bacterial systems to study its structure-function relationships. The protein features conserved retroviral elements, including a surface (SU) subunit for receptor binding and a transmembrane (TM) subunit mediating membrane fusion. Its expression is linked to certain cancers, autoimmune diseases, and neuroinflammatory disorders, making it a focus for diagnostic or therapeutic research.
Research on ERVK-6 aims to elucidate its role in human evolution and disease. For instance, HERV-K Env proteins may promote cell-cell fusion or immune evasion, potentially influencing tumor progression or neuronal damage. Recombinant ERVK-6 enables investigations into host-virus interactions, antibody production, and vaccine development. Additionally, it serves as a tool to explore HERV-K's contribution to genome plasticity and its dual role as both a symbiotic element and a pathogenic trigger.
Current studies emphasize its potential as a biomarker or target in conditions like amyotrophic lateral sclerosis (ALS) or multiple sclerosis, where HERV-K activation is observed. However, challenges remain in distinguishing its physiological functions from disease-associated activities. ERVK-6 recombinant protein thus represents a critical reagent for advancing understanding of endogenous retroviruses in human biology and pathology.
×
