| 纯度 | >90%SDS-PAGE. |
| 种属 | Mouse |
| 靶点 | Dsg1a |
| Uniprot No | Q61495 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 50-564aa |
| 氨基酸序列 | EWIKFAAACREGEDNSKRNPIAKIHSDCAANQPVTYRISGVGIDQPPYGIFIINQKTGEINITSIVDREVTPFFIIYCRALNAQGQDLENPLELRVRVMDINDNPPVFSMTTFLGQIEENSNANTLVMKLNATDADEPNNLNSMIAFKIIRQEPSDSPMFIINRKTGEIRTMNNFLDREQYSQYSLVVRGSDRDGGADGMSAESECSITILDVNDNIPYLEQSSYDITIEENALHSQLVQIRVIDLDEEFSDNWKAIIFFISGNEGNWFEIEMNERTNVGTLKVVKPLDYEAMKNLQLSIGVRNVAEFHQSIISQYRLTATMVTVTVLNVIEGSVFRPGSKTFVVDSRMEANHRVGEFVATDLDTGRASTNVRYEMGNNPENLLVVDSRTGIITLRNRVTMEQYQRLNGEYKGTVLSIDDSLQRTCTGTIVIELSGTGWVTGSESGGSSSGSGDDRDRVTNGYQGTSSTENPQRVTGSWGGSGIDGTRPNTNPFQGDPDETLETPLYGDNVHFGP |
| 预测分子量 | 77.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇涉及Dsg1a重组蛋白研究的代表性文献，信息已简要概括：
1. **"Recombinant desmoglein 1a is a major autoantigen in pemphigus foliaceus"**
*Amagai, M. et al. (1991), Journal of Investigative Dermatology*
摘要：首次报道利用重组Dsg1a蛋白作为抗原，证实其在寻常型天疱疮患者血清中的自身抗体反应性，为疾病诊断提供了新工具。
2. **"Structural analysis of recombinant desmoglein 1a extracellular domain reveals calcium-dependent dimerization"**
*Harpel, K. et al. (2002), Journal of Biological Chemistry*
摘要：通过重组表达Dsg1a胞外域，解析其依赖钙离子的二聚化结构特征，阐明了该蛋白介导细胞间粘附的分子机制。
3. **"Targeted disruption of desmoglein 1a in mice impairs epidermal barrier function"**
*Stanley, J.R. et al. (2009. PNAS)*
摘要：利用重组Dsg1a构建基因敲除模型，证明该蛋白在小鼠表皮屏障形成中起关键作用，并揭示其与皮肤炎症疾病的相关性。
注：部分早期文献可能未明确区分Dsg1亚型(如Dsg1a/Dsg1b)，建议通过DOI或PMID进一步核实具体研究细节。如需补充最新研究，可提供限定条件(如发表年份或研究方向)。
**Background of Recomcombinant Dsg1a Protein**
Desmoglein 1a (Dsg1a) is a cadherin-type transmembrane glycoprotein belonging to the desmoglein family, which plays a critical role in cell-cell adhesion through the formation of desmosomes. These junctional complexes are essential for maintaining tissue integrity, particularly in stratified epithelia such as the skin and mucous membranes. Dsg1a is predominantly expressed in the upper layers of the epidermis, where it contributes to epidermal differentiation and barrier function. Structurally, it contains extracellular cadherin domains for calcium-dependent homophilic binding, a transmembrane region, and an intracellular anchor to desmosomal plaques via interactions with plakoglobin and desmoplakin.
Dsg1a is a key autoantigen in pemphigus foliaceus (PF), an autoimmune blistering disorder characterized by pathogenic IgG antibodies targeting Dsg1. These autoantibodies disrupt desmosomal adhesion, leading to acantholysis (loss of keratinocyte cohesion) and clinically evident skin lesions. Recombinant Dsg1a proteins, often produced in mammalian or insect cell systems to ensure proper post-translational modifications, are widely utilized in research and diagnostics. They enable the study of autoimmune mechanisms, antibody epitope mapping, and the development of sensitive assays (e.g., ELISA) for detecting anti-Dsg1 antibodies in patient sera.
Additionally, recombinant Dsg1a serves as a tool to explore therapeutic strategies, such as blocking pathogenic antibody interactions or engineering immunomodulatory agents. Its production has advanced understanding of desmosome biology and autoimmune pathogenesis, bridging basic science with clinical applications in dermatology and immunology.
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