| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CELA3B |
| Uniprot No | P08861 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 29-270aa |
| 氨基酸序列 | VVNGEDAVPYSWPWQVSLQYEKSGSFYHTCGGSLIAPDWVVTAGHCISSSRTYQVVLGEYDRAVKEGPEQVIPINSGDLFVHPLWNRSCVACGNDIALIKLSRSAQLGDAVQLASLPPAGDILPNETPCYITGWGRLYTNGPLPDKLQEALLPVVDYEHCSRWNWWGSSVKKTMVCAGGDIRSGCNGDSGGPLNCPTEDGGWQVHGVTSFVSAFGCNTRRKPTVFTRVSAFIDWIEETIASH |
| 预测分子量 | 30.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CELA3B重组蛋白的3篇参考文献示例(注：以下内容为模拟生成，实际文献需通过学术数据库验证)：
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1. **文献名称**: "Recombinant Expression and Functional Characterization of Human CELA3B in a Pancreatic Cancer Model"
**作者**: Zhang Y, et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化了重组人CELA3B蛋白，通过体外酶活实验证实其对弹性蛋白底物的水解活性。进一步在胰腺癌细胞模型中，发现CELA3B通过降解细胞外基质促进癌细胞侵袭，提示其可能成为治疗靶点。
2. **文献名称**: "Structural and Biochemical Analysis of Recombinant CELA3B Reveals Substrate Specificity Determinants"
**作者**: Lee H, et al.
**摘要**: 通过昆虫细胞系统表达重组CELA3B，结合X射线晶体学解析其三维结构，揭示了底物结合口袋的关键氨基酸残基。酶动力学实验表明，CELA3B对含疏水残基的肽链具有高选择性，为设计特异性抑制剂奠定基础。
3. **文献名称**: "Optimization of CELA3B Production in Pichia pastoris for Industrial Enzymatic Applications"
**作者**: Brown K, et al.
**摘要**: 研究优化了毕赤酵母中CELA3B的分泌表达条件，采用发酵罐规模化生产并获得高纯度蛋白。酶稳定性测试表明，重组CELA3B在宽pH范围内保持活性，适用于洗涤剂和食品加工工业。
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**注意**：以上文献为示例，实际引用时请通过PubMed、Google Scholar等平台检索真实文献，并核对作者、标题及摘要准确性。若研究领域较新，可扩展关键词如“CELA3B recombinant expression”、“CELA3B enzymatic activity”进行检索。
CELA3B (Chymotrypsin-like Elastase Family Member 3B) is a serine protease belonging to the chymotrypsin family, primarily expressed in the pancreas. It is encoded by the CELA3B gene and plays a role in digestive processes by cleaving peptide bonds, particularly those adjacent to hydrophobic amino acids. Structurally, it shares homology with other pancreatic elastases but exhibits distinct substrate specificity. CELA3B is synthesized as an inactive zymogen (proenzyme) and activated proteolytically in the duodenum, contributing to nutrient breakdown. Dysregulation of CELA3B has been linked to pancreatic disorders, including pancreatitis and pancreatic cancer, where its overexpression may promote tumor progression through extracellular matrix remodeling or signaling pathway modulation.
Recombinant CELA3B protein is produced using biotechnological platforms (e.g., E. coli, mammalian cells) to enable functional studies. This engineered protein retains enzymatic activity and is purified via affinity chromatography, often with tags like His-tag for detection. Researchers utilize recombinant CELA3B to investigate its physiological roles, substrate interactions, and pathological mechanisms. Recent studies explore its potential as a biomarker for pancreatic diseases or a therapeutic target. Additionally, structural analysis of recombinant CELA3B aids in drug design, particularly for inhibitors that could mitigate its activity in disease contexts. Its study also contributes to understanding protease networks in digestion and inflammation. As an isoform of CELA3A, comparative analyses of CELA3B help elucidate functional divergence within this enzyme family.
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