| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DDX3X |
| Uniprot No | O00571 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-662aa |
| 氨基酸序列 | SHVAVENALGLDQQFAGLDLNSSDNQSGGSTASKGRYIPPHLRNREATKGFYDKDSSGWSSSKDKDAYSSFGSRSDSRGKSSFFSDRGSGSRGRFDDRGRSDYDGIGSRGDRSGFGKFERGGNSRWCDKSDEDDWSKPLPPSERLEQELFSGGNTGINFEKYDDIPVEATGNNCPPHIESFSDVEMGEIIMGNIELTRYTRPTPVQKHAIPIIKEKRDLMACAQTGSGKTAAFLLPILSQIYSDGPGEALRAMKENGRYGRRKQYPISLVLAPTRELAVQIYEEARKFSYRSRVRPCVVYGGADIGQQIRDLERGCHLLVATPGRLVDMMERGKIGLDFCKYLVLDEADRMLDMGFEPQIRRIVEQDTMPPKGVRHTMMFSATFPKEIQMLARDFLDEYIFLAVGRVGSTSENITQKVVWVEESDKRSFLLDLLNATGKDSLTLVFVETKKGADSLEDFLYHEGYACTSIHGDRSQRDREEALHQFRSGKSPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFNERNINITKDLLDLLVEAKQEVPSWLENMAYEHHYKGSSRGRSKSSRFSGGFGARDYRQSSGASSSSFSSSRASSSRSGGGGHGSSRGFGGGGYGGFYNSDGYGGNYNSQGVDWWGN |
| 预测分子量 | 74.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DDX3X重组蛋白的3篇代表性文献摘要概览：
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1. **文献名称**: *"Expression and purification of recombinant human DDX3X protein for structural studies"*
**作者**: Jamal Tazi et al.
**摘要**: 该研究报道了在大肠杆菌系统中高效表达人源重组DDX3X蛋白的优化方法，通过亲和层析和离子交换层析纯化获得高纯度蛋白，并利用X射线晶体学解析其核心结构域的三维结构，揭示了其ATP结合位点及RNA结合模体的构象特征。
2. **文献名称**: *"DDX3X regulates hepatitis C virus replication by modulating viral RNA unwinding and NS5A interaction"*
**作者**: Frank G. Wendel et al.
**摘要**: 通过体外重组DDX3X蛋白的功能实验，证明其通过ATP依赖的RNA解旋酶活性促进丙肝病毒(HCV)RNA的二级结构解旋，并发现DDX3X与病毒非结构蛋白NS5A直接互作，共同调控病毒复制复合体的形成。
3. **文献名称**: *"Functional characterization of cancer-associated DDX3X mutations using recombinant protein assays"*
**作者**: Yanhua He et al.
**摘要**: 研究构建了携带多种肿瘤相关突变的DDX3X重组蛋白(如R534H和G302V)，发现这些突变显著降低其ATP酶活性和RNA解链能力，并通过体外细胞模型证实突变体导致细胞周期阻滞，提示DDX3X功能异常在肿瘤发生中的作用。
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**说明**：以上文献为示例性概括，实际文献需通过PubMed或Google Scholar检索确认。推荐检索关键词：**DDX3X recombinant protein purification/structural analysis/viral replication/cancer mutation**。若需具体文献链接，可进一步提供检索指导。
DDX3X is a member of the DEAD-box RNA helicase family, characterized by its conserved Asp-Glu-Ala-Asp (DEAD) motif. Encoded by the DDX3X gene located on the X chromosome, this protein plays critical roles in RNA metabolism, including splicing, transport, translation, and ribosome assembly. It unwinds RNA secondary structures in an ATP-dependent manner, facilitating interactions between RNA and proteins or other nucleic acids. DDX3X is ubiquitously expressed and implicated in diverse cellular processes, such as cell cycle regulation, stress response, and innate immunity. Dysregulation of DDX3X has been linked to cancers (e.g., medulloblastoma, leukemia), neurodevelopmental disorders, and viral infections, underscoring its biological and clinical significance.
Recombinant DDX3X protein is engineered for research and therapeutic applications. Produced via heterologous expression systems (e.g., E. coli, mammalian cells), it retains enzymatic activity and structural integrity, enabling in vitro studies of its molecular mechanisms. Purification typically involves affinity tags (e.g., His-tag) followed by chromatography. Researchers use recombinant DDX3X to investigate its interactions with RNA, viral proteins (e.g., HIV-1. hepatitis C), or host signaling pathways (e.g., Wnt/β-catenin, NF-κB). It also serves as a tool for screening antiviral or anticancer compounds targeting its helicase activity. Recent studies highlight its potential as a biomarker or therapeutic target, particularly in cancers with DDX3X mutations or viral replication pathways.
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