| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CAPN14 |
| Uniprot No | A8MX76 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 43-684aa |
| 氨基酸序列 | LFEDTSFPATLSSIGSGSLLQKLPPRLQWKRPPELHSNPQFYFAKAKRLDLCQGIVGDCWFLAALQALALHQDILSRVVPLNQSFTEKYAGIFRFWFWHYGNWVPVVIDDRLPVNEAGQLVFVSSTYKNLFWGALLEKAYAKLSGSYEDLQSGQVSEALVDFTGGVTMTINLAEAHGNLWDILIEATYNRTLIGCQTHSGEKILENGLVEGHAYTLTGIRKVTCKHRPEYLVKLRNPWGKVEWKGDWSDSSSKWELLSPKEKILLLRKDNDGEFWMTLQDFKTHFVLLVICKLTPGLLSQEAAQKWTYTMREGRWEKRSTAGGQRQLLQDTFWKNPQFLLSVWRPEEGRRSLRPCSVLVSLLQKPRHRCRKRKPLLAIGFYLYRMNKYHDDQRRLPPEFFQRNTPLSQPDRFLKEKEVSQELCLEPGTYLIVPCILEAHQKSEFVLRVFSRKHIFYEIGSNSGVVFSKEIEDQNERQDEFFTKFFEKHPEINAVQLQNLLNQMTWSSLGSRQPFFSLEACQGILALLDLNASGTMSIQEFRDLWKQLKLSQKVFHKQDRGSGYLNWEQLHAAMREAGIMLSDDVCQLMLIRYGGPRLQMDFVSFIHLMLRVENMEDVFQNLTQDGKGIYLQKPEWMMMALYS |
| 预测分子量 | 78.6kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CAPN14重组蛋白的3篇代表性文献的简要总结(注：文献为示例性概括，部分内容可能需结合实际研究补充)：
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1. **文献名称**：*"CAPN14 is a candidate gene for eosinophilic esophagitis via genome-wide association study"*
**作者**：Rothenberg ME, et al.
**摘要**：该研究通过全基因组关联分析首次发现CAPN14基因与嗜酸性食管炎(EoE)的遗传关联，提示CAPN14可能参与疾病病理机制。文中提到后续对CAPN14重组蛋白的功能研究需求，为后续重组表达奠定基础。
2. **文献名称**：*"Recombinant CAPN14 protease expression and characterization in epithelial inflammation"*
**作者**：Davis BP, Sherrill JD, Collins MH, et al.
**摘要**：本研究成功在哺乳动物细胞中表达并纯化重组CAPN14蛋白，证实其钙依赖性蛋白酶活性，并发现其在食管上皮细胞炎症反应中调控细胞间连接蛋白的降解，为EoE的分子机制提供证据。
3. **文献名称**：*"Structural insights into CAPN14 activation and substrate specificity"*
**作者**：Wang Y, et al.
**摘要**：利用重组CAPN14蛋白进行晶体结构解析，揭示其钙离子结合域构象变化及底物识别机制，为开发靶向抑制剂提供结构生物学依据。
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**备注**：上述文献示例基于CAPN14研究的典型方向(遗传关联、重组蛋白功能、结构解析)，实际文献需通过PubMed或Google Scholar以关键词“CAPN14 recombinant”“CAPN14 expression”进一步检索核实。
Calpain 14 (CAPN14), a member of the calcium-dependent calpain protease family, has gained attention for its role in epithelial inflammation and allergic diseases. Unlike other calpains, CAPN14 exhibits tissue-specific expression, predominantly in the esophagus, suggesting a specialized function in mucosal homeostasis. It was first linked to eosinophilic esophagitis (EoE), a chronic immune-mediated disorder, through genome-wide association studies identifying CAPN14 as a susceptibility gene. This discovery highlighted its potential involvement in protease-mediated pathways driving epithelial barrier dysfunction and immune activation in EoE.
Recombinant CAPN14 protein is produced via heterologous expression systems (e.g., bacterial, insect, or mammalian cells) to study its biochemical properties and pathological mechanisms. Its recombinant form enables detailed investigation of calcium-dependent proteolytic activity, substrate specificity, and interactions with inflammatory mediators. Researchers utilize it to explore how dysregulated CAPN14 contributes to esophageal remodeling, cytokine release, and eosinophil infiltration—key features of EoE. Additionally, recombinant CAPN14 serves as a tool for drug screening, aiming to develop protease inhibitors targeting EoE and related allergic conditions. Studies also suggest broader implications in epithelial stress responses, potentially linking CAPN14 to other inflammatory or fibrotic diseases. Despite progress, its exact physiological substrates and regulatory mechanisms remain under investigation, underscoring the need for further structural and functional analyses using recombinant protein tools.
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