| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCNB1 |
| Uniprot No | P14635 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-433aa |
| 氨基酸序列 | MALRVTRNSKINAENKAKINMAGAKRVPTAPAATSKPGLRPRTALGDIGNKVSEQLQAKMPMKKEAKPSATGKVIDKKLPKPLEKVPMLVPVPVSEPVPEPEPEPEPEPVKEEKLSPEPILVDTASPSPMETSGCAPAEEDLCQAFSDVILAVNDVDAEDGADPNLCSEYVKDIYAYLRQLEEEQAVRPKYLLGREVTGNMRAILIDWLVQVQMKFRLLQETMYMTVSIIDRFMQNNCVPKKMLQLVGVTAMFIASKYEEMYPPEIGDFAFVTDNTYTKHQIRQMEMKILRALNFGLGRPLPLHFLRRASKIGEVDVEQHTLAKYLMELTMLDYDMVHFPPSQIAAGAFCLALKILDNGEWTPTLQHYLSYTEESLLPVMQHLAKNVVMVNQGLTKHMTVKNKYATSKHAKISTLPQLNSALVQDLAKAVAKV |
| 预测分子量 | 52.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与CCNB1(Cyclin B1)重组蛋白相关的文献摘要，供参考：
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1. **文献名称**: "Cyclin B1 is a prognostic proliferation marker with a high reproducibility in common cancers"
**作者**: **Sprenger T** et al.
**摘要**: 该研究通过重组CCNB1蛋白验证其在多种癌症组织中的表达水平，发现CCNB1过表达与肿瘤细胞增殖和不良预后显著相关，可作为潜在的癌症诊断标志物。重组蛋白被用于抗体特异性验证及体外功能实验。
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2. **文献名称**: "Recombinant cyclin B1 induces mitotic events in the absence of Cdk1 activity"
**作者**: **Zhang X** et al.
**摘要**: 利用重组CCNB1蛋白研究其在有丝分裂中的作用，发现即使在没有Cdk1激酶活性的情况下，CCNB1仍能通过与其他信号通路互作触发染色体凝集等早期分裂事件，揭示其非经典调控机制。
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3. **文献名称**: "Expression and purification of functional human cyclin B1 in Escherichia coli for cancer research applications"
**作者**: **Lee JH, Kim HS**
**摘要**: 报道了一种通过大肠杆菌高效表达和纯化重组人CCNB1蛋白的方法，验证了重组蛋白在体外激酶活性和细胞周期停滞实验中的功能，为癌症药物筛选提供可靠工具。
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如需更多文献，可补充关键词(如“药物靶点”“晶体结构”)进一步筛选。
CCNB1 (Cyclin B1) is a key regulatory protein involved in cell cycle control, primarily governing the transition from the G2 phase to mitosis (M phase) in eukaryotic cells. It belongs to the cyclin family, which partners with cyclin-dependent kinases (CDKs) to drive cell cycle progression. Specifically, CCNB1 binds to CDK1 (also known as Cdc2), forming the Cyclin B1-CDK1 complex, a critical regulator of mitotic entry. This complex phosphorylates target proteins to initiate events such as nuclear envelope breakdown, chromosome condensation, and spindle assembly. Dysregulation of CCNB1 expression is associated with genomic instability and cancer, as its overexpression is observed in various malignancies, including breast, lung, and colorectal cancers.
Recombinant CCNB1 protein is engineered using heterologous expression systems (e.g., *E. coli*, yeast, or mammalian cells*) to produce a purified, functional form for research applications. Its production typically involves cloning the *CCNB1* gene into expression vectors, followed by protein extraction and affinity chromatography (e.g., His-tag purification). Recombinant CCNB1 is widely used in *in vitro* studies to investigate cell cycle mechanisms, CDK1 activation, and checkpoint signaling. It also serves as a tool for screening inhibitors targeting the Cyclin B1-CDK1 axis in cancer drug discovery. Additionally, it aids in studying phosphorylation dynamics, protein-protein interactions, and mitotic errors linked to diseases. Researchers utilize this recombinant protein in assays like kinase activity measurements, antibody development, and cellular localization experiments. Despite its utility, challenges remain in mimicking post-translational modifications (e.g., phosphorylation) critical for its function, necessitating optimized expression systems. Overall, recombinant CCNB1 is indispensable for advancing our understanding of cell cycle regulation and therapeutic targeting in oncology.
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