| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCNK |
| Uniprot No | O75909 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-354aa |
| 氨基酸序列 | MKENKENSSPSVTSANLDHTKPCWYWDKKDLAHTPSQLEGLDPATEARYRREGARFIFDVGTRLGLHYDTLATGIIYFHRFYMFHSFKQFPRYVTGACCLFLAGKVEETPKKCKDIIKTARSLLNDVQFGQFGDDPKEEVMVLERILLQTIKFDLQVEHPYQFLLKYAKQLKGDKNKIQKLVQMAWTFVNDSLCTTLSLQWEPEIIAVAVMYLAGRLCKFEIQEWTSKPMYRRWWEQFVQDVPVDVLEDICHQILDLYSQGKQQMPHHTPHQLQQPPSLQPTPQVPQVQQSQPSQSSEPSQPQQKDPLILLQGWACRQPATHLLPSPLEDSLLCPRPFPHPACLQLGGWGGQPG |
| 预测分子量 | 42.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CCNK重组蛋白的示例参考文献(内容为模拟概括，建议通过专业数据库核实)：
1. **文献名称**：*Cyclin K Regulates CDK12-Mediated RNA Polymerase II Phosphorylation*
**作者**：Smith A, et al.
**摘要**：研究通过重组CCNK蛋白与CDK12的体外共表达，揭示了CCNK在调控RNA聚合酶II羧基末端结构域磷酸化中的关键作用，证实其参与转录延伸的分子机制。
2. **文献名称**：*Expression and Functional Characterization of Recombinant Cyclin K in DNA Damage Response*
**作者**：Lee B, et al.
**摘要**：利用昆虫细胞系统表达纯化CCNK重组蛋白，发现其与CDK13结合后激活激酶活性，并参与DNA损伤修复通路，提示其在基因组稳定性中的功能。
3. **文献名称**：*Structural Insights into Cyclin K-CDK12 Complex Assembly*
**作者**：Zhang X, et al.
**摘要**：通过重组CCNK蛋白的结晶学研究，解析了其与CDK12的复合物结构，阐明了二者相互作用的关键结构域，为靶向该复合物的药物设计提供基础。
4. **文献名称**：*Optimization of Recombinant Cyclin K Production in E. coli for High-Throughput Screening*
**作者**：Wang Y, et al.
**摘要**：报道了在大肠杆菌中高效表达可溶性CCNK重组蛋白的工艺优化，并应用于激酶抑制剂的高通量筛选，验证了其在药物开发中的应用潜力。
**注意**：以上内容为模拟示例，实际文献需通过PubMed、Web of Science等平台检索关键词(如“CCNK recombinant protein”、“Cyclin K expression”)获取。
**Background of CCNK (Cyclin K) Recombinant Protein**
CCNK (Cyclin K) is a member of the cyclin family, which plays critical roles in regulating cell cycle progression and transcriptional processes. Cyclins function as regulatory subunits of cyclin-dependent kinases (CDKs), forming complexes that drive cell cycle transitions and modulate gene expression. CCNK specifically interacts with CDK12 and CDK13. two kinases involved in transcriptional elongation, RNA splicing, and DNA damage response. Unlike classical cyclins (e.g., Cyclin D or Cyclin B), CCNK is not strictly cell cycle-dependent but is ubiquitously expressed, highlighting its broader regulatory functions in transcription and genome stability.
Structurally, CCNK contains conserved cyclin domains that enable CDK binding and kinase activation. Its role in facilitating phosphorylation of RNA polymerase II and components of the DNA repair machinery underscores its importance in coordinating transcription with DNA repair pathways. Dysregulation of CCNK has been implicated in cancer, neurodegenerative disorders, and viral infections, making it a target for therapeutic research.
Recombinant CCNK protein is produced using genetic engineering techniques, often in *E. coli* or mammalian expression systems, to ensure high purity and functionality. This protein retains the ability to bind CDK12/13 and activate their kinase activity, enabling in vitro studies on transcriptional regulation, drug screening, and mechanistic investigations. Researchers utilize CCNK recombinant protein to dissect its interactions with CDKs, explore its role in DNA damage responses, and develop inhibitors for diseases linked to its overexpression or malfunction. Its application spans structural biology, cancer biology, and drug discovery, underscoring its significance in both basic and translational research.
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