| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TNKS1BP1 |
| Uniprot No | Q9C0C2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1729aa |
| 氨基酸序列 | MKVSTLRESSAMASPLPREMEEELVPTGSEPGDTRAKPPVKPKPRALPAKPALPAKPSLLVPVGPRPPRGPLAELPSARKMNMLAGPQPYGGSKRPLPFAPRPAVEASTGGEATQETGKEEAGKEEPPPLTPPARCAAPGGVRKAPAPFRPASERFAATTVEEILAKMEQPRKEVLASPDRLWGSRLTFNHDGSSRYGPRTYGTTTAPRDEDGSTLFRGWSQEGPVKSPAECREEHSKTPEERSLPSDLAFNGDLAKAASSELPADISKPWIPSSPAPSSENGGPASPGLPAEASGSGPGSPHLHPPDKSSPCHSQLLEAQTPEASQASPCPAVTPSAPSAALPDEGSRHTPSPGLPAEGAPEAPRPSSPPPEVLEPHSLDQPPATSPRPLIEVGELLDLTRTFPSGGEEEAKGDAHLRPTSLVQRRFSEGVLQSPSQDQEKLGGSLAALPQGQGSQLALDRPFGAESNWSLSQSFEWTFPTRPSGLGVWRLDSPPPSPITEASEAAEAAEAGNLAVSSREEGVSQQGQGAGSAPSGSGSSWVQGDDPSMSLTQKGDGESQPQFPAVPLEPLPTTEGTPGLPLQQAEERYESQEPLAGQESPLPLATREAALPILEPVLGQEQPAAPDQPCVLFADAPEPGQALPVEEEAVTLARAETTQARTEAQDLCRASPEPPGPESSSRWLDDLLASPPPSGGGARRGAGAELKDTQSPSTCSEGLLGWSQKDLQSEFGITGDPQPSSFSPSSWCQGASQDYGLGGASPRGDPGLGERDWTSKYGQGAGEGSTREWASRCGIGQEEMEASSSQDQSKVSAPGVLTAQDRVVGKPAQLGTQRSQEADVQDWEFRKRDSQGTYSSRDAELQDQEFGKRDSLGTYSSRDVSLGDWEFGKRDSLGAYASQDANEQGQDLGKRDHHGRYSSQDADEQDWEFQKRDVSLGTYGSRAAEPQEQEFGKSAWIRDYSSGGSSRTLDAQDRSFGTRPLSSGFSPEEAQQQDEEFEKKIPSVEDSLGEGSRDAGRPGERGSGGLFSPSTAHVPDGALGQRDQSSWQNSDASQEVGGHQERQQAGAQGPGSADLEDGEMGKRGWVGEFSLSVGPQREAAFSPGQQDWSRDFCIEASERSYQFGIIGNDRVSGAGFSPSSKMEGGHFVPPGKTTAGSVDWTDQLGLRNLEVSSCVGSGGSSEARESAVGQMGWSGGLSLRDMNLTGCLESGGSEEPGGIGVGEKDWTSDVNVKSKDLAEVGEGGGHSQARESGVGQTDWSGVEAGEFLKSRERGVGQADWTPDLGLRNMAPGAVCSPGESKELGVGQMDWGNNLGLRDLEVTCDPDSGGSQGLRGCGVGQMDWTQDLAPQNVELFGAPSEAREHGVGGVSQCPEPGLRHNGSLSPGLEARDPLEARELGVGETSGPETQGEDYSSSSLEPHPADPGMETGEALSFGASPGRCPARPPPSGSQGLLEEMLAASSSKAVARRESAASGLGGLLEEEGAGAGAAQEEVLEPGRDSPPSWRPQPDGEASQTEDVDGTWGSSAARWSDQGPAQTSRRPSQGPPARSPSQDFSFIEDTEILDSAMYRSRANLGRKRGHRAPVIRPGGTLGLSEAADSDAHLFQDSTEPRASRVPSSDEEVVEEPQSRRTRMSLGTKGLKVNLFPGLSPSALKAKLRPRNRSAEEGELAESKSSQKESAVQRSKSCKVPGLGKPLTLPPKPEKSSGSEGSSPNWLQALKLKKKKV |
| 预测分子量 | 181,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TNKS1BP1重组蛋白的示例参考文献(注：以下内容为假设性示例，实际文献需通过学术数据库验证)：
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1. **文献名称**: *"Recombinant TNKS1BP1 Expression and Its Role in Wnt Signaling Modulation"*
**作者**: Zhang Y, et al.
**摘要**: 本研究成功在大肠杆菌中表达了重组TNKS1BP1蛋白，并验证其通过结合Tankyrase抑制Wnt/β-catenin信号通路的活性，为靶向治疗癌症提供了潜在策略。
2. **文献名称**: *"Structural Characterization of TNKS1BP1 Recombinant Protein via Cryo-EM"*
**作者**: Smith J, et al.
**摘要**: 利用冷冻电镜解析了重组TNKS1BP1的三维结构，揭示了其与Tankyrase相互作用的特异性结构域，为设计小分子抑制剂奠定基础。
3. **文献名称**: *"TNKS1BP1 Recombinant Protein Suppresses Telomerase Activity in Cancer Cells"*
**作者**: Lee S, et al.
**摘要**: 通过体外实验证明，重组TNKS1BP1蛋白通过调控端粒酶复合体稳定性，抑制多种癌细胞系的增殖，提示其作为抗癌靶点的潜力。
4. **文献名称**: *"High-Yield Purification of TNKS1BP1 Recombinant Protein for Functional Studies"*
**作者**: Patel R, et al.
**摘要**: 开发了一种高效纯化TNKS1BP1重组蛋白的层析方法，并验证其在细胞迁移实验中的功能，为后续机制研究提供可靠工具。
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**建议**：实际文献可通过PubMed、Google Scholar等平台以关键词“TNKS1BP1 recombinant protein”或“Tankyrase 1 Binding Protein 1 expression”检索，或结合其别名(如Tab1)扩展搜索。
TNKS1BP1 (Tankyrase 1 Binding Protein 1), also known as TAB182. is a multifunctional scaffold protein that interacts with tankyrase 1 (TNKS1), a member of the poly(ADP-ribose) polymerase (PARP) family. This interaction is mediated through its C-terminal sterile alpha motif (SAM) domain, which binds to the ankyrin repeat clusters of TNKS1. TNKS1BP1 plays a regulatory role in diverse cellular processes, including telomere maintenance, Wnt/β-catenin signaling, and genomic stability. It acts as a negative modulator of TNKS1 activity by competing with other substrates for binding, thereby influencing PARsylation-dependent pathways critical for cancer progression and stem cell regulation.
Recombinant TNKS1BP1 protein is engineered for in vitro studies to dissect its molecular interactions and therapeutic potential. Typically produced in bacterial or mammalian expression systems, the purified protein retains functional domains required for binding TNKS1 and other partners. Its applications span mechanistic studies of tankyrase-mediated processes, drug screening for PARP inhibitors, and structural analyses to map interaction interfaces. Researchers also utilize it to explore TNKS1BP1’s role in diseases; for instance, dysregulation of TNKS1-TNKS1BP1 axis is implicated in colorectal cancer (via Wnt pathway) and neurodegenerative disorders. However, challenges remain in resolving its full interactome and context-dependent functions. Current efforts focus on optimizing recombinant variants (e.g., truncated forms or tagged versions) to enhance solubility and experimental utility, supporting translational research targeting tankyrase-related pathologies.
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