| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ITF1 |
| Uniprot No | P15923 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-654aa |
| 氨基酸序列 | MNQPQRMAPV GTDKELSDLL DFSMMFPLPV TNGKGRPASL AGAQFGGSGL EDRPSSGSWG SGDQSSSSFD PSRTFSEGTH FTESHSSLSS STFLGPGLGG KSGERGAYAS FGRDAGVGGL TQAGFLSGEL ALNSPGPLSP SGMKGTSQYY PSYSGSSRRR AADGSLDTQP KKVRKVPPGL PSSVYPPSSG EDYGRDATAY PSAKTPSSTY PAPFYVADGS LHPSAELWSP PGQAGFGPML GGGSSPLPLP PGSGPVGSSG SSSTFGGLHQ HERMGYQLHG AEVNGGLPSA SSFSSAPGAT YGGVSSHTPP VSGADSLLGS RGTTAGSSGD ALGKALASIY SPDHSSNNFS SSPSTPVGSP QGLAGTSQWP RAGAPGALSP SYDGGLHGLQ SKIEDHLDEA IHVLRSHAVG TAGDMHTLLP GHGALASGFT GPMSLGGRHA GLVGGSHPED GLAGSTSLMH NHAALPSQPG TLPDLSRPPD SYSGLGRAGA TAAASEIKRE EKEDEENTSA ADHSEEEKKE LKAPRARTSP DEDEDDLLPP EQKAEREKER RVANNARERL RVRDINEAFK ELGRMCQLHL NSEKPQTKLL ILHQAVSVIL NLEQQVRERN LNPKAACLKR REEEKVSGVV GDPQMVLSAP HPGLSEAHNP AGHM |
| 预测分子量 | 67,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ITF1重组蛋白的模拟参考文献示例(建议通过学术数据库核实具体文献)：
---
1. **文献名称**: *"High-Yield Expression and Purification of Recombinant Human ITF1 in a Prokaryotic System"*
**作者**: Zhang, Y. et al.
**摘要**: 本研究报道了利用大肠杆菌表达系统高效生产重组人ITF1蛋白的优化策略，通过密码子优化和亲和层析技术获得高纯度蛋白，并验证了其生物活性。
2. **文献名称**: *"ITF1 Recombinant Protein Enhances Intestinal Epithelial Repair via PI3K/Akt Signaling Pathway"*
**作者**: Chen, L. et al.
**摘要**: 研究证明重组ITF1蛋白通过激活PI3K/Akt通路促进肠上皮细胞迁移和增殖，为治疗肠道黏膜损伤提供了实验依据。
3. **文献名称**: *"Structural and Functional Characterization of Recombinant ITF1 in Inflammatory Bowel Disease Models"*
**作者**: Gupta, R. et al.
**摘要**: 通过核磁共振解析ITF1重组蛋白的三维结构，并在小鼠结肠炎模型中证实其减轻炎症、加速黏膜修复的作用。
4. **文献名称**: *"Development of a Stable Mammalian Cell Line for ITF1 Recombinant Protein Production"*
**作者**: Kim, S. et al.
**摘要**: 构建了稳定表达ITF1重组蛋白的CHO细胞系，优化了培养条件以提高产量，并验证了其糖基化修饰对功能的影响。
---
**注意**：上述文献为模拟示例，实际研究中请通过 **PubMed、Web of Science 或 Google Scholar** 等平台检索真实文献。若ITF1指其他蛋白(如转录因子)，建议结合具体研究领域调整关键词。
ITF-1 (Intestinal Trefoil Factor 1), also known as TFF3 (Trefoil Factor Family 3), is a small secreted protein belonging to the trefoil factor family. It is primarily expressed in goblet cells of the intestinal mucosa and plays a critical role in mucosal protection and repair. The protein’s name derives from its characteristic three-loop "trefoil" structure stabilized by disulfide bonds, which confers resistance to enzymatic degradation and extreme pH conditions—an essential feature for functioning in the harsh gastrointestinal environment.
Biologically, ITF-1 promotes epithelial restitution by enhancing cell migration and suppressing apoptosis during injury. It interacts with mucins to stabilize the mucus layer, maintaining barrier integrity against pathogens and irritants. Its expression is upregulated in response to mucosal damage, inflammation, or infections, suggesting a self-repair mechanism in gastrointestinal disorders like inflammatory bowel disease (IBD) or peptic ulcers.
Recombinant ITF-1 is produced via genetic engineering, typically using bacterial (e.g., *E. coli*) or mammalian expression systems. The recombinant protein retains native bioactivity and is utilized in research to study mucosal healing pathways or as a potential therapeutic agent. Preclinical studies highlight its efficacy in accelerating ulcer healing and reducing inflammation in animal models. However, challenges remain in optimizing delivery systems for clinical use due to its rapid clearance and instability *in vivo*.
Current research focuses on ITF-1’s therapeutic potential in gastrointestinal diseases, cancer (due to its role in epithelial cell survival), and tissue engineering. Its recombinant form offers a standardized tool for mechanistic studies and drug development, bridging translational gaps in mucosal health research.
×
