| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CRTC2 |
| Uniprot No | Q53ET0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-693aa |
| 氨基酸序列 | ATSGANGPG SATASASNPR KFSEKIALQK QRQAEETAAF EEVMMDIGST RLQAQKLRLA YTRSSHYGGS LPNVNQIGSG LAEFQSPLHS PLDSSRSTRH HGLVERVQRD PRRMVSPLRR YTRHIDSSPY SPAYLSPPPE SSWRRTMAWG NFPAEKGQLF RLPSALNRTS SDSALHTSVM NPSPQDTYPG PTPPSILPSR RGGILDGEMD PKVPAIEENL LDDKHLLKPW DAKKLSSSSS RPRSCEVPGI NIFPSPDQPA NVPVLPPAMN TGGSLPDLTN LHFPPPLPTP LDPEETAYPS LSGGNSTSNL THTMTHLGIS RGMGLGPGYD APGLHSPLSH PSLQSSLSNP NLQASLSSPQ PQLQGSHSHP SLPASSLARH VLPTTSLGHP SLSAPALSSS SSSSSTSSPV LGAPSYPAST PGASPHHRRV PLSPLSLLAG PADARRSQQQ LPKQFSPTMS PTLSSITQGV PLDTSKLSTD QRLPPYPYSS PSLVLPTQPH TPKSLQQPGL PSQSCSVQSS GGQPPGRQSH YGTPYPPGPS GHGQQSYHRP MSDFNLGNLE QFSMESPSAS LVLDPPGFSE GPGFLGGEGP MGGPQDPHTF NHQNLTHCSR HGSGPNIILT GDSSPGFSKE IAAALAGVPG FEVSAAGLEL GLGLEDELRM EPLGLEGLNM LSDPCALLPD PAVEESFRSD RLQ |
| 预测分子量 | 73,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CRTC2重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**: *"CRTC2 is a key mediator of AMPK-regulated skeletal muscle metabolism"*
**作者**: Li Y, et al.
**摘要**: 该研究揭示了CRTC2在AMPK信号通路调控骨骼肌能量代谢中的作用。通过重组CRTC2蛋白的体外实验，发现其磷酸化状态直接调控糖异生和脂质氧化相关基因的表达，为代谢疾病治疗提供新靶点。
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2. **文献名称**: *"Structural basis of CREB recognition by the CRTC2 coactivator"*
**作者**: Wang C, et al.
**摘要**: 本研究利用重组CRTC2蛋白进行X射线晶体学分析，解析了CRTC2与转录因子CREB的复合物结构，揭示了二者结合的关键结构域，阐明了其在基因转录调控中的分子机制。
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3. **文献名称**: *"CRTC2重组蛋白在肝癌细胞增殖中的功能研究"*
**作者**: 张某某, 等.
**摘要**: 通过体外表达CRTC2重组蛋白，研究发现其在肝癌细胞中异常激活Wnt/β-catenin通路，促进肿瘤增殖和转移，提示CRTC2可能作为肝癌治疗的潜在生物标志物。
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(注：以上文献信息为示例，实际文献需通过PubMed、Web of Science等数据库检索确认。)
CRTC2 (CREB-regulated transcription coactivator 2) is a key regulatory protein involved in cellular energy metabolism and stress response pathways. As a member of the CRTC family, it functions as a coactivator for CREB (cAMP-response element-binding protein), enhancing transcription of target genes by bridging CREB and the basal transcriptional machinery. CRTC2 is particularly notable for its role in gluconeogenesis regulation, where it activates genes like *PGC-1α* and *G6Pase* in response to hormonal signals such as glucagon.
Under normal conditions, CRTC2 is sequestered in the cytoplasm through phosphorylation by AMP-activated protein kinase (AMPK) and salt-inducible kinases (SIKs). Cellular stress, nutrient deprivation, or cAMP-inducing signals trigger its dephosphorylation and nuclear translocation. This dynamic regulation links CRTC2 to metabolic homeostasis, making it a critical player in diabetes and obesity research. Dysregulation of CRTC2 has been associated with insulin resistance and hepatic glucose overproduction in type 2 diabetes.
Recombinant CRTC2 proteins are engineered using expression systems like *E. coli* or mammalian cells, often fused with tags (e.g., GST, His-tag) for purification and detection. These tools enable studies on post-translational modifications, protein-protein interactions (e.g., with CREB or regulatory kinases), and screening for metabolic disease therapeutics. Recent research also explores its involvement in cancer progression and aging-related metabolic disorders, highlighting its broad therapeutic potential. Efficient production of functional CRTC2 recombinant protein remains essential for dissecting its complex regulatory networks and developing targeted therapies.
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