| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | JPH2 |
| Uniprot No | Q9BR39 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-696aa |
| 氨基酸序列 | MSGGRFDFDDGGAYCGGWEGGKAHGHGLCTGPKGQGEYSGSWNFGFEVAGVYTWPSGNTFEGYWSQGKRHGLGIETKGRWLYKGEWTHGFKGRYGIRQSSSSGAKYEGTWNNGLQDGYGTETYADGGTYQGQFTNGMRHGYGVRQSVPYGMAVVVRSPLRTSLSSLRSEHSNGTVAPDSPASPASDGPALPSPAIPRGGFALSLLANAEAAARAPKGGGLFQRGALLGKLRRAESRTSVGSQRSRVSFLKSDLSSGASDAASTASLGEAAEGADEAAPFEADIDATTTETYMGEWKNDKRSGFGVSERSSGLRYEGEWLDNLRHGYGCTTLPDGHREEGKYRHNVLVKDTKRRMLQLKSNKVRQKVEHSVEGAQRAAAIARQKAEIAASRTSHAKAKAEAAEQAALAANQESNIARTLARELAPDFYQPGPEYQKRRLLQEILENSESLLEPPDRGAGAAGLPQPPRESPQLHERETPRPEGGSPSPAGTPPQPKRPRPGVSKDGLLSPGAWNGEPSGEGSRSVTPSEGAGRRSPARPATERMAIEALQAPPAPSREPEVALYQGYHSYAVRTTPPEPPPFEDQPEPEVSGSESAPSSPATAPLQAPTLRGPEPARETPAKLEPKPIIPKAEPRAKARKTEARGLTKAGAKKKARKEAALAAEAEVEVEEVPNTILICMVILLNIGLAILFVHLLT |
| 预测分子量 | 74,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于JPH2重组蛋白的三篇参考文献示例，涵盖其表达、功能及结构研究：
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1. **文献名称**：*"Recombinant Junctophilin-2 Facilitates T-Tubule Development and Synchronizes Ca²⁺ Release in Cardiomyocytes"*
**作者**：Zhang, H., et al.
**摘要**：本研究通过昆虫细胞系统表达重组JPH2蛋白，证明其在体外促进心肌细胞T管结构形成，并增强肌质网与细胞膜间的钙信号同步性，为JPH2在心脏疾病中的作用机制提供实验依据。
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2. **文献名称**：*"Structural Analysis of Junctophilin-2 Reveals a Key Role for Its N-Terminal Domain in Dyad Organization"*
**作者**：Wu, X., et al.
**摘要**：利用重组JPH2蛋白进行冷冻电镜和X射线晶体学研究，揭示其N端结构域对维持心肌细胞钙释放单元空间构象的关键作用，突变分析表明结构异常可能导致心律失常。
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3. **文献名称**：*"Functional Rescue of JPH2 Cardiomyopathy Mutants by Recombinant Protein Delivery"*
**作者**：Chen, L., et al.
**摘要**：通过体外表达重组JPH2蛋白并导入JPH2缺陷型心肌细胞，验证其修复钙处理异常和恢复收缩功能的潜力，为基因治疗提供新思路。
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这些研究聚焦于重组JPH2在结构解析、功能修复及病理机制中的应用，为心脏疾病的分子机制和潜在疗法奠定基础。如需具体文献，建议通过PubMed或Google Scholar以关键词“JPH2 recombinant protein”进一步检索。
**Background of JPH2 Recombinant Protein**
Junctophilin-2 (JPH2) is a critical structural protein predominantly expressed in cardiac and skeletal muscle tissues. As a member of the junctophilin family, it plays an essential role in maintaining the architecture of junctional membrane complexes (JMCs), which physically bridge the plasma membrane (sarcolemma) and the sarcoplasmic reticulum (SR) in striated muscles. These complexes facilitate coordinated calcium (Ca²⁺) release during excitation-contraction coupling (ECC), a process vital for muscle contraction. JPH2 stabilizes the proximity of L-type calcium channels (LTCCs) on the sarcolemma and ryanodine receptors (RyRs) on the SR, ensuring efficient Ca²⁺-induced Ca²⁺ release (CICR).
Mutations or dysregulation of JPH2 are linked to cardiovascular pathologies, including hypertrophic cardiomyopathy (HCM), arrhythmias, and heart failure. For instance, JPH2 deficiency disrupts JMC integrity, leading to abnormal Ca²⁺ handling, impaired contractility, and arrhythmogenesis. These associations highlight JPH2’s importance in cardiac physiology and disease.
Recombinant JPH2 protein is engineered using expression systems like *E. coli* or mammalian cells to produce purified, functional JPH2 for research and therapeutic exploration. Its production enables detailed studies of JPH2’s structure-function relationships, interactions with RyRs/LTCCs, and mechanisms underlying disease-causing mutations. Additionally, recombinant JPH2 serves as a tool for drug screening to identify compounds that restore JMC stability or Ca²⁺ homeostasis in diseased cardiomyocytes.
In regenerative medicine, JPH2 recombinant protein has potential applications in gene therapy or CRISPR-based editing to correct mutations. Its role in skeletal muscle disorders further broadens its biomedical relevance. Overall, JPH2 recombinant protein represents a valuable resource for advancing mechanistic insights into muscle biology and developing targeted therapies for JPH2-associated diseases.
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