| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SRFBP1 |
| Uniprot No | Q8NEF9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-429aa |
| 氨基酸序列 | AQPGTLNLN NEVVKMRKEV KRIRVLVIRK LVRSVGRLKS KKGTEDALLK NQRRAQRLLE EIHAMKELKP DIVTKSALGD DINFEKIFKK PDSTATERAI ARLAVHPLLK KKIDVLKAAV QAFKEARQNV AEVESSKNAS EDNHSENTLY SNDNGSNLQR EATVISEQKV KETKILAKKP IHNSKEKIAK MEHGPKAVTI ANSPSKPSEK DSVVSLESQK TPADPKLKTL SQTKKNKGSD SSLSGNSDGG EEFCEEEKEY FDDSTEERFY KQSSMSEDSD SGDDFFIGKV RRTRKKESSC HSSVKEQKPL EKVFLKEDTG ETHGDTRNDK IKPSTETRKL ESVFFHSLSG SKSSRRNFKE QAPKTRSLDF PQNEPQIKNQ FNKKLSGRLE NTKQQLQLPL HPSWEASRRR KEQQSNIAVF QGKKITFDD |
| 预测分子量 | 48,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟的关于SRFBP1重组蛋白的参考文献示例(实际文献可能需要根据具体数据库检索确认)：
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1. **文献名称**：*Cloning and Functional Analysis of Recombinant SRFBP1 in Cancer Cell Proliferation*
**作者**：Zhang L. et al.
**摘要**：研究通过克隆SRFBP1基因并在HEK293细胞中表达重组蛋白，发现SRFBP1通过调控血清反应因子(SRF)信号通路抑制肿瘤细胞增殖，提示其潜在的抑癌功能。
2. **文献名称**：*Expression and Purification of SRFBP1 Recombinant Protein for Structural Studies*
**作者**：Smith J.R., Patel K.
**摘要**：开发了一种高效的大肠杆菌表达系统，用于纯化高活性SRFBP1重组蛋白，并通过X射线晶体学解析其三维结构，为功能机制研究提供基础。
3. **文献名称**：*SRFBP1 Interacts with MYOCD in Cardiac Hypertrophy Regulation*
**作者**：Wang Y. et al.
**摘要**：利用重组SRFBP1蛋白进行体外结合实验，证实其与心肌肥厚关键因子MYOCD的相互作用，并揭示其在心脏病理重塑中的调控作用。
4. **文献名称**：*Role of SRFBP1 in Neuronal Differentiation: Insights from Recombinant Protein Overexpression*
**作者**：Kim S. et al.
**摘要**：通过神经元细胞模型过表达重组SRFBP1.发现其促进神经突生长并激活MAPK通路，表明其在神经发育中的潜在功能。
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**注意**：以上为模拟摘要，实际文献需通过PubMed、Web of Science等平台检索关键词(如"SRFBP1 recombinant"、"SRFBP1 expression")获取。若研究较新或小众，建议结合相关领域(如SRF信号通路、重组蛋白技术)扩大检索范围。
Serum response factor binding protein 1 (SRFBP1) is a conserved eukaryotic protein implicated in transcriptional regulation, cell cycle control, and signal transduction. Initially identified as an interacting partner of serum response factor (SRF), a key transcription factor regulating genes involved in proliferation, differentiation, and apoptosis, SRFBP1 modulates SRF-mediated transcriptional activity through direct binding. Structurally, it contains a C-terminal coiled-coil domain critical for protein-protein interactions and nuclear localization signals, enabling its shuttling between cytoplasm and nucleus.
Recombinant SRFBP1 protein is engineered to facilitate functional studies, typically expressed in bacterial (e.g., E. coli) or mammalian systems with affinity tags (e.g., His-tag, GST) for purification. Its production enables in vitro investigations into molecular mechanisms, such as SRF-dependent gene regulation, post-translational modifications, and interaction networks. Studies suggest SRFBP1 may act as a scaffold protein, coordinating crosstalk between SRF and other signaling pathways like MAPK/ERK, potentially influencing cellular responses to stress, growth signals, or cytoskeletal changes.
Emerging evidence links SRFBP1 dysregulation to human diseases. It shows altered expression in cancers (e.g., hepatocellular carcinoma, glioblastoma) and cardiovascular disorders, where it may affect cell migration, angiogenesis, or apoptosis. Recombinant SRFBP1 serves as a vital tool for structural studies, antibody development, and high-throughput screening for therapeutic agents targeting SRF-related pathologies. Ongoing research aims to clarify its tissue-specific roles and validate its potential as a diagnostic biomarker or therapeutic target.
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