| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DEFB118 |
| Uniprot No | Q96PH6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-123aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSYSGEKKCWNRSGHCRKQCKDGEAVKDT CKNLRACCIPSNEDHRRVPATSPTPLSDSTPGIIDDILTVRFTTDYFEVS SKKDMVEESEAGRGTETSLPNVHHSS |
| 预测分子量 | 14 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DEFB118重组蛋白的3篇代表性文献摘要概括：
1. **文献名称**: *"Recombinant human β-defensin 118 promotes sperm motility and survival in vitro"*
**作者**: Li Y, et al.
**摘要**: 研究通过大肠杆菌系统成功表达并纯化DEFB118重组蛋白，证明其在体外能显著增强精子运动能力并抑制氧化应激诱导的精子凋亡，提示其在男性不育治疗中的潜在应用。
2. **文献名称**: *"Expression and functional characterization of recombinant DEFB118 in antimicrobial activity"*
**作者**: Chen Z, et al.
**摘要**: 通过哺乳动物细胞表达系统获得功能性DEFB118重组蛋白，发现其对革兰氏阴性菌(如大肠杆菌)和真菌具有广谱抗菌活性，并揭示其作用机制与细胞膜破坏相关。
3. **文献名称**: *"DEFB118重组蛋白对女性生殖道免疫微环境的调节作用"*
**作者**: Wang X, et al.
**摘要**: 研究利用昆虫杆状病毒系统表达DEFB118.证实其可通过激活TLR4/NF-κB通路增强子宫内膜上皮细胞的先天免疫应答，可能参与生殖道感染防御。
注：以上文献为示例，实际文献需通过PubMed/CNKI等数据库检索。建议结合具体研究方向补充关键词(如“重组表达”、“抗菌”或“生殖健康”)筛选最新研究。
DEFB118 recombinant protein is derived from the human DEFB118 gene, which encodes a member of the beta-defensin family. Beta-defensins are small cationic peptides characterized by six conserved cysteine residues forming three disulfide bonds, contributing to their antimicrobial and immunomodulatory functions. DEFB118. specifically expressed in the male reproductive tract (notably the epididymis), is implicated in host defense and sperm maturation. It exhibits broad-spectrum antimicrobial activity against bacteria, fungi, and viruses by disrupting microbial membranes through electrostatic interactions. Additionally, DEFB118 may regulate immune responses by recruiting immune cells or modulating cytokine production.
The recombinant form of DEFB118 is typically produced using expression systems like *E. coli* or mammalian cells, followed by purification to ensure bioactivity. Its study helps elucidate mechanisms of innate immunity in reproductive health, particularly in protecting against urogenital infections and maintaining sperm functionality. Research also explores its potential role in male infertility, as altered DEFB118 expression correlates with impaired sperm motility and viability. Beyond reproductive biology, DEFB118's antimicrobial properties inspire therapeutic development for multidrug-resistant infections. Current investigations focus on structure-function relationships, signaling pathways, and clinical applications, positioning it as a dual-purpose molecule bridging reproductive physiology and antimicrobial defense strategies.
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