| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DDX39A |
| Uniprot No | O00148 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-427aa |
| 氨基酸序列 | AEQDVENDLLDYDEEEEPQAPQESTPAPPKKDIKGSYVSIHSSGFRDFLLKPELLRAIVDCGFEHPSEVQHECIPQAILGMDVLCQAKSGMGKTAVFVLATLQQIEPVNGQVTVLVMCHTRELAFQISKEYERFSKYMPSVKVSVFFGGLSIKKDEEVLKKNCPHVVVGTPGRILALVRNRSFSLKNVKHFVLDECDKMLEQLDMRRDVQEIFRLTPHEKQCMMFSATLSKDIRPVCRKFMQDPMEVFVDDETKLTLHGLQQYYVKLKDSEKNRKLFDLLDVLEFNQVIIFVKSVQRCMALAQLLVEQNFPAIAIHRGMAQEERLSRYQQFKDFQRRILVATNLFGRGMDIERVNIVFNYDMPEDSDTYLHRVARAGRFGTKGLAITFVSDENDAKILNDVQDRFEVNVAELPEEIDISTYIEQSR |
| 预测分子量 | 55.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **DDX39A重组蛋白** 的3篇虚构参考文献示例(实际文献需通过数据库检索)：
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1. **文献名称**: *Structural and functional analysis of human DDX39A reveals its role in mRNA nuclear export*
**作者**: Smith J, Lee H, et al.
**摘要**: 本研究通过重组表达纯化人源DDX39A蛋白，解析了其ATP依赖的RNA解旋酶活性及晶体结构，发现其通过与TAP/NXF1复合物相互作用参与mRNA出核转运，为理解DDX39A在RNA代谢中的机制提供依据。
2. **文献名称**: *DDX39A interacts with the spliceosome to regulate pre-mRNA splicing in cancer cells*
**作者**: Zhang Y, Wang X, et al.
**摘要**: 通过重组DDX39A蛋白的体外结合实验和RNA免疫沉淀技术，揭示了DDX39A与剪接体组分(如U2AF65)的相互作用，表明其在调控前体mRNA剪接及肿瘤细胞增殖中的关键作用。
3. **文献名称**: *DDX39A deficiency impairs antiviral immunity by disrupting RIG-I signaling pathway*
**作者**: Tanaka K, Chen L, et al.
**摘要**: 利用重组DDX39A蛋白进行体外功能补偿实验，发现DDX39A通过结合RIG-I并促进其ATP水解活性，增强宿主抗病毒天然免疫应答，其缺失导致病毒RNA识别能力下降。
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建议通过 **PubMed/Google Scholar** 搜索真实文献，关键词：*DDX39A recombinant protein, DDX39A function, DEAD-box helicase*。
**Background of DDX39A Recombinant Protein**
DDX39A (DEAD-box helicase 39A) is a member of the DEAD-box RNA helicase family, characterized by the conserved Asp-Glu-Ala-Asp (DEAD) motif. This protein plays a critical role in RNA metabolism, including pre-mRNA splicing, nuclear export of mRNA, and ribosome biogenesis. It utilizes ATP hydrolysis to unwind RNA secondary structures or dissociate RNA-protein complexes, facilitating dynamic RNA processing and transport. DDX39A is predominantly localized in the nucleus and is involved in transcriptional regulation, particularly in coordinating mRNA synthesis and export. Its interaction with components of the TREX complex highlights its importance in coupling transcription with RNA export machinery.
The recombinant DDX39A protein is engineered using heterologous expression systems, such as *E. coli* or mammalian cell cultures, to produce a purified, biologically active form. This allows researchers to study its enzymatic activities, structural properties, and interactions with nucleic acids or partner proteins *in vitro*. Recombinant DDX39A is instrumental in dissecting molecular mechanisms underlying RNA processing disorders, viral replication (as some viruses hijack host RNA helicases), and diseases linked to DDX39A dysregulation, such as cancers and neurodegenerative conditions.
Structurally, DDX39A contains conserved helicase core domains and flexible termini that mediate substrate binding. Studies using recombinant protein have revealed its ATPase and helicase activities are modulated by RNA/DNA substrates and cofactors. Furthermore, DDX39A shares homology with its paralog DDX39B (UAP56), but functional distinctions are emerging; for instance, DDX39A may compensate for DDX39B loss in mRNA export. Ongoing research leverages recombinant DDX39A to explore its therapeutic potential, including targeting its activity in diseases with aberrant RNA metabolism or as a biomarker for diagnostics.
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