| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DDX56 |
| Uniprot No | Q9NY93 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-547aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMEDSEAL GFEHMGLDPR LLQAVTDLGW SRPTLIQEKA IPLALEGKDL LARARTGSGK TAAYAIPMLQ LLLHRKATGP VVEQAVRGLV LVPTKELARQ AQSMIQQLAT YCARDVRVAN VSAAEDSVSQ RAVLMEKPDV VVGTPSRILS HLQQDSLKLR DSLELLVVDE ADLLFSFGFE EELKSLLCHL PRIYQAFLMS ATFNEDVQAL KELILHNPVT LKLQESQLPG PDQLQQFQVV CETEEDKFLL LYALLKLSLI RGKSLLFVNT LERSYRLRLF LEQFSIPTCV LNGELPLRSR CHIISQFNQG FYDCVIATDA EVLGAPVKGK RRGRGPKGDK ASDPEAGVAR GIDFHHVSAV LNFDLPPTPE AYIHRAGRTA RANNPGIVLT FVLPTEQFHL GKIEELLSGE NRGPILLPYQ FRMEEIEGFR YRCRDAMRSV TKQAIREARL KEIKEELLHS EKLKTYFEDN PRDLQLLRHD LPLHPAVVKP HLGHVPDYLV PPALRGLVRP HKKRKKLSSS CRKAKRAKSQ NPLRSFKHKG KKFRPTAKPS |
| 预测分子量 | 64 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DDX56重组蛋白的3篇参考文献的简要总结(基于模拟数据，仅供参考)：
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1. **文献名称**：*Recombinant DDX56 exhibits ATP-dependent RNA helicase activity and facilitates viral RNA replication*
**作者**：Zhang Y, et al.
**摘要**：本研究在大肠杆菌中成功表达并纯化了重组DDX56蛋白，通过体外实验证实其具有ATP酶活性，并能解旋双链RNA。进一步发现DDX56与丙肝病毒(HCV)的RNA复制复合体相互作用，提示其在病毒复制中的潜在作用。
2. **文献名称**：*Crystal structure of human DDX56 reveals the mechanism of RNA binding and ATP hydrolysis*
**作者**：Li X, et al.
**摘要**：作者利用昆虫细胞系统表达重组人源DDX56蛋白，解析了其高分辨率晶体结构，揭示了其DEAD-box结构域中ATP和RNA结合的关键位点，为理解DDX56在RNA代谢中的分子机制提供了结构基础。
3. **文献名称**：*DDX56 regulates innate immune response by interacting with mitochondrial antiviral signaling protein (MAVS)*
**作者**：Wang C, et al.
**摘要**：通过哺乳动物细胞表达的重组DDX56蛋白，研究发现其通过与MAVS蛋白的相互作用负调控I型干扰素信号通路，表明DDX56在抗病毒先天免疫中的新功能。
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如需实际文献，建议在PubMed或Google Scholar中检索关键词“DDX56 recombinant protein”或“DDX56 expression and function”。
DDX56. a member of the DEAD-box RNA helicase family, is a conserved ATP-dependent enzyme critical in RNA metabolism. Characterized by its signature "DEAD" motif (Asp-Glu-Ala-Asp), this protein unwinds RNA secondary structures, facilitating processes like ribosome biogenesis, pre-mRNA splicing, and viral replication. It localizes predominantly to the nucleolus, where it participates in rRNA processing and ribosome assembly. Studies also implicate DDX56 in cell cycle regulation and nucleocytoplasmic transport of viral RNAs.
Recombinant DDX56 refers to the protein artificially expressed in heterologous systems (e.g., *E. coli* or mammalian cells) for functional studies. Its production enables precise investigation of molecular mechanisms, including ATPase/helicase activities and RNA-binding properties. Researchers leverage recombinant DDX56 to dissect its role in viral life cycles; for instance, it interacts with hepatitis C virus (HCV), influenza, and SARS-CoV-2 RNAs, enhancing viral replication. This makes it a potential target for antiviral therapies.
In cancer biology, DDX56 overexpression correlates with tumor progression in malignancies like glioblastoma and hepatocellular carcinoma, suggesting its utility as a diagnostic biomarker or therapeutic target. Recombinant proteins are pivotal in drug discovery, enabling high-throughput screens for inhibitors. Structural studies using recombinant DDX56. such as cryo-EM or X-ray crystallography, reveal conformational changes during RNA unwinding, informing rational drug design.
Despite its established roles, DDX56’s regulatory networks and disease-specific pathways remain incompletely understood. Recombinant variants, including mutants or tagged forms (e.g., GFP/His-tagged), help map functional domains and protein interactomes. Ongoing research aims to clarify its dual roles in host cell homeostasis and viral pathogenesis, balancing therapeutic potential against risks of disrupting essential cellular functions. This positions DDX56 as a compelling subject for both basic research and translational applications.
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