| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CYP2E1 |
| Uniprot No | P05181 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 29-493aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSSWNLPPG PFPLPIIGNL FQLELKNIPK SFTRLAQRFG PVFTLYVGSQ RMVVMHGYKA VKEALLDYKD EFSGRGDLPA FHAHRDRGII FNNGPTWKDI RRFSLTTLRN YGMGKQGNES RIQREAHFLL EALRKTQGQP FDPTFLIGCA PCNVIADILF RKHFDYNDEK FLRLMYLFNE NFHLLSTPWL QLYNNFPSFL HYLPGSHRKV IKNVAEVKEY VSERVKEHHQ SLDPNCPRDL TDCLLVEMEK EKHSAERLYT MDGITVTVAD LFFAGTETTS TTLRYGLLIL MKYPEIEEKL HEEIDRVIGP SRIPAIKDRQ EMPYMDAVVH EIQRFITLVP SNLPHEATRD TIFRGYLIPK GTVVVPTLDS VLYDNQEFPD PEKFKPEHFL NENGKFKYSD YFKPFSTGKR VCAGEGLARM ELFLLLCAIL QHFNLKPLVD PKDIDLSPIH IGFGCIPPRY KLCVIPRS |
| 预测分子量 | 56 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Expression and Purification of Functional Human CYP2E1 in Escherichia coli"**
- **作者**: Gonzalez FJ, et al.
- **摘要**: 该研究报道了利用大肠杆菌系统高效表达人源CYP2E1重组蛋白的方法，优化了表达条件与纯化步骤，并通过光谱分析和酶活实验验证了重组蛋白的活性，为后续药物代谢研究提供基础。
2. **"Structural Insights into CYP2E1 Interactions with Substrates and Inhibitors by X-ray Crystallography"**
- **作者**: Porubsky PR, et al.
- **摘要**: 通过X射线晶体学解析了重组CYP2E1蛋白与酒精代谢产物及抑制剂的复合物结构，揭示了其底物结合口袋的关键氨基酸残基，阐明了代谢选择性和抑制机制。
3. **"Functional Characterization of Recombinant CYP2E1 in a Baculovirus-Insect Cell System"**
- **作者**: Dai Y, et al.
- **摘要**: 利用杆状病毒-昆虫细胞系统表达CYP2E1重组蛋白，结合NADPH-P450还原酶共表达，系统评估了其对多种药物(如对乙酰氨基酚)的代谢能力及其在肝毒性中的作用。
4. **"Role of Recombinant CYP2E1 in Reactive Oxygen Species Generation and Oxidative Stress"**
- **作者**: Cederbaum AI, et al.
- **摘要**: 研究证实重组CYP2E1在代谢乙醇过程中产生大量活性氧(ROS)，并探讨了其与酒精性肝病中氧化损伤的关联，为靶向CYP2E1的抗氧化治疗提供依据。
(注：以上文献信息为示例性概括，实际引用需根据具体论文内容调整。)
CYP2E1 (cytochrome P450 2E1) is a key enzyme involved in the metabolism of xenobiotics and endogenous compounds. As a member of the cytochrome P450 superfamily, it plays a central role in oxidizing small hydrophobic molecules, including ethanol, acetaminophen, industrial solvents (e.g., benzene), and procarcinogens. Its activity is notable for generating reactive oxygen species (ROS) during catalytic cycles, linking it to oxidative stress and alcohol-related liver injury.
Recombinant CYP2E1 protein is produced through heterologous expression systems (e.g., E. coli, yeast, or insect cells) to study its structure-function relationships, substrate specificity, and interactions with inhibitors or drugs. Unlike some P450 isoforms, CYP2E1 exhibits high inducibility under physiological conditions such as fasting, diabetes, or chronic alcohol exposure, making its recombinant form valuable for modeling metabolic disorders. Structural studies using recombinant protein have revealed insights into its active site topology and mechanisms of substrate recognition, aiding drug design.
Research applications include screening drug metabolites, assessing chemical toxicity, and investigating CYP2E1's role in carcinogen activation (e.g., nitrosamines). Challenges in working with recombinant CYP2E1 include its membrane-associated nature, instability in purified form, and reliance on NADPH-cytochrome P450 reductase for activity in vitro. Recent advances in protein engineering and cryo-EM have enhanced its study, while controversies persist regarding its extrahepatic expression and polymorphic variations affecting individual drug responses. Current efforts focus on optimizing expression systems for higher yield and developing targeted inhibitors for therapeutic interventions in metabolic diseases.
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