| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CYB5R3 |
| Uniprot No | P00387 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 27-301aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMFQRST PAITLESPDI KYPLRLIDRE IISHDTRRFR FALPSPQHIL GLPVGQHIYL SARIDGNLVV RPYTPISSDD DKGFVDLVIK VYFKDTHPKF PAGGKMSQYL ESMQIGDTIE FRGPSGLLVY QGKGKFAIRP DKKSNPIIRT VKSVGMIAGG TGITPMLQVI RAIMKDPDDH TVCHLLFANQ TEKDILLRPE LEELRNKHSA RFKLWYTLDR APEAWDYGQG FVNEEMIRDH LPPPEEEPLV LMCGPPPMIQ YACLPNLDHV GHPTERCFVF |
| 预测分子量 | 34 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CYB5R3重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**：*"Functional characterization of human cytochrome b5 reductase (CYB5R3) variants associated with recessive congenital methemoglobinemia"*
**作者**：Shirabe K, et al.
**摘要**：该研究通过重组表达人类CYB5R3蛋白，分析了多种致病突变体(如R160Q、P275L)的酶活性及稳定性，揭示了突变导致酶功能缺陷的分子机制，为遗传性高铁血红蛋白血症的病理机制提供依据。
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2. **文献名称**：*"Expression and purification of functional recombinant human CYB5R3 in Escherichia coli: Application in drug metabolism studies"*
**作者**：Liao Y, et al.
**摘要**：研究报道了利用大肠杆菌系统高效表达可溶性CYB5R3重组蛋白的优化方法，并验证其在体外药物代谢模型中对细胞色素P450酶活性的协同作用，证明其潜在药理学应用价值。
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3. **文献名称**：*"Structural insights into the NADH-binding domain and dimerization of CYB5R3 by X-ray crystallography"*
**作者**：Binda C, et al.
**摘要**：通过X射线晶体学解析了重组CYB5R3蛋白的NADH结合域结构，阐明其催化反应中辅因子识别的关键残基，并揭示二聚化对酶活性调控的影响，为靶向设计抑制剂奠定基础。
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如需扩展检索，建议结合PubMed或Web of Science平台，以“CYB5R3 recombinant”为关键词筛选近年研究。
CYB5R3 (cytochrome b5 reductase 3) is a NADH-dependent enzyme that plays a critical role in redox metabolism. It catalyzes the reduction of cytochrome b5. facilitating electron transfer in biochemical pathways such as fatty acid desaturation, cholesterol synthesis, and drug/xenobiotic detoxification. This membrane-bound or soluble protein (depending on isoform) is particularly vital in erythrocytes, where it maintains hemoglobin iron in its functional ferrous state. Mutations in the CYB5R3 gene are linked to recessive congenital methemoglobinemia, a disorder characterized by impaired oxygen transport due to excessive methemoglobin accumulation.
Recombinant CYB5R3 protein is produced via heterologous expression systems (e.g., E. coli, mammalian cells) to study its enzymatic mechanisms, structural properties, and pathological variants. Its purified form enables in vitro assays to assess reductase activity, screen potential therapeutic agents, or model genetic deficiencies. Researchers also leverage recombinant CYB5R3 to explore its broader roles in nitric oxide metabolism, cancer progression, and oxidative stress responses. Notably, soluble isoforms have therapeutic potential for enzyme replacement therapy in methemoglobinemia, while membrane-associated isoforms are investigated for lipid metabolism regulation. Recent advances in protein engineering aim to enhance its stability and delivery efficiency for clinical applications. As a versatile tool, recombinant CYB5R3 continues to bridge gaps between genetic studies, molecular pathophysiology, and translational medicine in hematologic and metabolic diseases.
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