| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPID |
| Uniprot No | Q08752 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-370aa |
| 氨基酸序列 | MSHPSPQAKPSNPSNPRVFFDVDIGGERVGRIVLELFADIVPKTAENFRALCTGEKGIGHTTGKPLHFKGCPFHRIIKKFMIQGGDFSNQNGTGGESIYGEKFEDENFHYKHDREGLLSMANAGRNTNGSQFFITTVPTPHLDGKHVVFGQVIKGIGVARILENVEVKGEKPAKLCVIAECGELKEGDDGGIFPKDGSGDSHPDFPEDADIDLKDVDKILLITEDLKNIGNTFFKSQNWEMAIKKYAEVLRYVDSSKAVIETADRAKLQPIALSCVLNIGACKLKMSNWQGAIDSCLEALELDPSNTKALYRRAQGWQGLKEYDQALADLKKAQGIAPEDKAIQAELLKVKQKIKAQKDKEKAVYAKMFA |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是围绕PPID(肽基脯氨酰异构酶D)重组蛋白的示例性参考文献及摘要概括(注：文献信息为模拟示例，非真实存在)：
1. **文献名称**：*"Recombinant PPID Expression and Purification for Structural Analysis"*
**作者**：Smith J. et al.
**摘要**：研究报道了利用大肠杆菌系统高效表达重组PPID蛋白的优化方法，通过亲和层析纯化获得高纯度蛋白，并利用X射线晶体学解析其三维结构，揭示了其与底物结合的关键位点。
2. **文献名称**：*"Functional Role of PPID in Mitochondrial Protein Folding"*
**作者**：Li Y. et al.
**摘要**：通过重组PPID蛋白体外功能实验，证明其作为分子伴侣参与线粒体蛋白质折叠过程，并发现其活性缺失与细胞氧化应激反应增强相关，提示PPID在维持线粒体功能中的重要性。
3. **文献名称**：*"PPID Knockdown and Recombinant Rescue in Cancer Cell Models"*
**作者**：Garcia R. et al.
**摘要**：研究利用重组PPID蛋白回补实验，验证了PPID在癌细胞凋亡调控中的作用，表明其通过调控Bcl-2家族蛋白稳定性影响化疗耐药性，为靶向PPID的抗癌策略提供依据。
4. **文献名称**：*"High-Throughput Screening of PPID Inhibitors Using Recombinant Protein"*
**作者**：Chen L. et al.
**摘要**：基于重组PPID蛋白建立高通量药物筛选平台，鉴定出多个小分子抑制剂，并通过体外酶活实验和细胞模型验证其抑制效果，为开发PPID相关疾病治疗药物奠定基础。
**注意**：以上文献为生成示例，实际研究中请通过PubMed、Web of Science等平台检索真实文献。
**Background of PPID Recombinant Protein**
PPID (Peptidyl-prolyl cis-trans isomerase D), also known as Cyclophilin D, is a mitochondrial protein belonging to the immunophilin family. It functions as a key regulator of the mitochondrial permeability transition pore (mPTP), a critical channel implicated in cell death pathways. PPID exhibits peptidyl-prolyl isomerase activity, facilitating conformational changes in target proteins by catalyzing cis-trans isomerization of proline residues. This activity is vital for modulating mitochondrial integrity, calcium homeostasis, and responses to oxidative stress. Dysregulation of PPID is linked to pathologies such as ischemia-reperfusion injury, neurodegenerative disorders, and cancer, highlighting its role in cellular survival and apoptosis.
Recombinant PPID protein is engineered using expression systems like *E. coli* or mammalian cells, enabling large-scale production for research and therapeutic exploration. The recombinant form retains the functional domain of PPID, allowing studies on its interaction with mPTP components, ligands (e.g., cyclosporine A), and mechanisms underlying mitochondrial dysfunction. Its applications span structural biology, drug screening for mPTP inhibitors, and elucidating disease mechanisms.
Recent advancements in protein engineering have improved the stability and purity of recombinant PPID, facilitating high-resolution studies such as X-ray crystallography and cryo-EM. Despite progress, challenges remain in understanding tissue-specific PPID interactions and developing isoform-selective modulators. Overall, PPID recombinant protein serves as a pivotal tool for decoding mitochondrial biology and designing targeted therapies for metabolic and degenerative diseases.
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