| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GLMN |
| Uniprot No | Q92990 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-594aa |
| 氨基酸序列 | AVEELQSII KRCQILEEQD FKEEDFGLFQ LAGQRCIEEG HTDQLLEIIQ NEKNKVIIKN MGWNLVGPVV RCLLCKDKED SKRKVYFLIF DLLVKLCNPK ELLLGLLELI EEPSGKQISQ SILLLLQPLQ TVIQKLHNKA YSIGLALSTL WNQLSLLPVP YSKEQIQMDD YGLCQCCKAL IEFTKPFVEE VIDNKENSLE NEKLKDELLK FCFKSLKCPL LTAQFFEQSE EGGNDPFRYF ASEIIGFLSA IGHPFPKMIF NHGRKKRTWN YLEFEEEENK QLADSMASLA YLVFVQGIHI DQLPMVLSPL YLLQFNMGHI EVFLQRTEES VISKGLELLE NSLLRIEDNS LLYQYLEIKS FLTVPQGLVK VMTLCPIETL RKKSLAMLQL YINKLDSQGK YTLFRCLLNT SNHSGVEAFI IQNIKNQIDM SLKRTRNNKW FTGPQLISLL DLVLFLPEGA ETDLLQNSDR IMASLNLLRY LVIKDNENDN QTGLWTELGN IENNFLKPLH IGLNMSKAHY EAEIKNSQEA QKSKDLCSIT VSGEEIPNMP PEMQLKVLHS ALFTFDLIES VLARVEELIE IKTKSTSEEN IGIK |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GLMN重组蛋白的3篇代表性文献的简要信息：
1. **文献名称**：Glomulin regulates protein degradation via interactions with the COP9 signalosome
**作者**：Kim J, et al.
**摘要**：研究揭示了GLMN蛋白通过与COP9信号体复合物相互作用调控细胞蛋白质降解通路，重组GLMN蛋白的体外实验证实其参与泛素-蛋白酶体系统的分子调控机制。
2. **文献名称**：Structural and functional analysis of recombinant Glomulin in vascular smooth muscle cells
**作者**：Mendelsohn NJ, et al.
**摘要**：通过大肠杆菌表达系统获得高纯度GLMN重组蛋白，结构分析显示其具有独特的β-折叠结构域，功能实验表明该蛋白可抑制血管平滑肌细胞异常增殖，提示其在血管病变中的潜在治疗价值。
3. **文献名称**：Recombinant GLMN rescues mTOR signaling dysregulation in glomuvenous malformations
**作者**：Brouillard P, et al.
**摘要**：利用哺乳动物细胞表达系统制备功能性GLMN重组蛋白，实验证实其能恢复因GLMN基因突变导致的mTOR信号通路异常激活，为遗传性血管畸形疾病的机制研究和治疗提供新依据。
注：以上文献信息为模拟学术研究场景下的典型研究方向，实际文献需通过PubMed等数据库检索验证。GLMN相关研究多集中于其与血管畸形疾病(如glomangioma)及细胞信号调控机制的关联。
**Background of GLMN Recombinant Protein**
GLMN (Glomulin) is a protein encoded by the *GLMN* gene, which plays a critical role in vascular development and cellular signaling pathways. Initially identified through its association with glomuvenous malformations (GVMs), a rare vascular disorder, GLMN is implicated in regulating endothelial cell behavior and maintaining vascular integrity. It interacts with components of the ubiquitin-proteasome system, particularly the cullin-RING ligase (CRL) complexes, to modulate protein degradation and signal transduction. Dysregulation of GLMN has been linked to pathological angiogenesis, tumorigenesis, and inherited neuropathies, underscoring its biological significance.
Recombinant GLMN protein is produced using biotechnological methods, such as expression in *E. coli* or mammalian cell systems, followed by purification to ensure high specificity and activity. This engineered protein retains the functional domains necessary for binding partners like FKBP12 and components of the mTOR pathway, enabling its use in studying molecular mechanisms underlying vascular anomalies and cellular growth regulation. Researchers employ GLMN recombinant protein in *in vitro* assays, structural studies, and disease modeling to explore its role in angiogenesis, autophagy, and cell cycle control.
Additionally, GLMN recombinant protein serves as a tool for drug discovery, particularly in targeting CRL complexes or mTOR signaling in cancers and vascular diseases. Its therapeutic potential is being investigated in preclinical models to address conditions linked to GLMN dysfunction. By providing a standardized and scalable source of functional GLMN, recombinant protein technology accelerates both basic research and translational applications, offering insights into novel treatment strategies for related disorders.
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