| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ACD |
| Uniprot No | Q96AP0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-458aa |
| 氨基酸序列 | MAGSGRLVLRPWIRELILGSETPSSPRAGQLLEVLQDAEAAVAGPSHAPDTSDVGATLLVSDGTHSVRCLVTREALDTSDWEEKEFGFRGTEGRLLLLQDCGVHVQVAEGGAPAEFYLQVDRFSLLPTEQPRLRVPGCNQDLDVQKKLYDCLEEHLSESTSSNAGLSLSQLLDEMREDQEHQGALVCLAESCLTLEGPCTAPPVTHWAASRCKATGEAVYTVPSSMLCISENDQLILSSLGPCQRTQGPELPPPDPALQDLSLTLIASPPSSPSSSGTPALPGHMSSEESGTSISLLPALSLAAPDPGQRSSSQPSPAICSAPATLTPRSPHASRTPSSPLQSCTPSLSPRSHVPSPHQALVTRPQKPSLEFKEFVGLPCKNRPPFPRTGATRGAQEPCSVWEPPKRHRDGSAFQYEYEPPCTSLCARVQAVRLPPQLMAWALHFLMDAQPGSEPTPM |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ACD重组蛋白的3篇文献示例(注：文献为虚构，仅供格式参考)：
1. **文献名称**：*Functional Characterization of Recombinant ACD5 Protein in Plant Apoptosis*
**作者**：Chen et al.
**摘要**：本研究通过原核表达系统纯化拟南芥ACD5重组蛋白，发现其通过调控线粒体膜通透性促进细胞程序性死亡，揭示了ACD5在植物发育与逆境响应中的关键作用。
2. **文献名称**：*ACD2 Recombinant Protein Enhances Disease Resistance in Crops*
**作者**：Li et al.
**摘要**：利用昆虫细胞表达体系制备高活性ACD2重组蛋白，证明其通过激活水杨酸信号通路显著提升水稻对病原菌的抗性，为农业抗病育种提供新策略。
3. **文献名称**：*Structural Analysis of ACD1 Recombinant Protein via Cryo-EM*
**作者**：Xu et al.
**摘要**：通过冷冻电镜解析ACD1重组蛋白的三维结构，阐明其与磷脂分子结合的活性位点，为设计靶向ACD蛋白的分子抑制剂奠定基础。
4. **文献名称**：*ACD7 Recombinant Protein as a Therapeutic Target in Cancer*
**作者**：Guo et al.
**摘要**：体外实验表明，哺乳动物细胞表达的ACD7重组蛋白能特异性诱导肿瘤细胞凋亡，且不影响正常细胞，提示其在癌症靶向治疗中的潜在应用价值。
(提示：如需真实文献，建议通过PubMed或Google Scholar检索“ACD recombinant protein”及相关关键词。)
ACD recombinant proteins are engineered biomolecules derived from the fusion or modification of functional domains, often involving the ACD (Adapter-Containing Domain) or other structurally/functionally defined motifs. These proteins are typically produced using recombinant DNA technology, where target gene sequences are inserted into expression vectors and expressed in host systems like *E. coli*, yeast, or mammalian cells. The term "ACD" may refer to specific domains critical for protein interactions, signaling, or structural roles, depending on the biological context. For instance, in apoptosis-related studies, ACD domains might mediate protein binding to regulate cell death pathways.
The development of ACD recombinant proteins aligns with advancements in structural biology and synthetic biology, enabling precise customization for research, therapeutic, or industrial applications. They are widely used to study protein-protein interactions, signal transduction mechanisms, or as tools in drug discovery. In therapeutics, such proteins may serve as biologics targeting diseases like cancer or autoimmune disorders, leveraging their specificity to modulate cellular processes. Industrial applications include enzyme engineering for biocatalysis or biosensors.
Production challenges include ensuring proper folding, post-translational modifications (e.g., phosphorylation), and scalability. Prokaryotic systems like *E. coli* offer cost-effective production but may lack eukaryotic modifications, whereas mammalian systems address this at higher costs. Innovations in cell-free systems or fusion tags (e.g., SUMO, GST) enhance solubility and purification efficiency.
ACD recombinant proteins exemplify the convergence of molecular design and biomanufacturing, driving progress in personalized medicine and biotechnology. Ongoing research focuses on optimizing expression platforms, enhancing functional fidelity, and expanding applications in emerging fields like CRISPR-based therapies or nanotechnology. Their versatility underscores their pivotal role in modern bioengineering and translational science.
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