| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ADM2 |
| Uniprot No | Q7Z4H4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 101-147aa |
| 氨基酸序列 | TQAQLLRVGC VLGTCQVQNL SHRLWQLMGP AGRQDSAPVD PSSPHSY |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Production and Characterization of Recombinant Human Adrenomedullin 2 (ADM2) Protein"**
- 作者：Li Y, et al.
- 摘要：研究描述了人源ADM2重组蛋白在大肠杆菌中的高效表达与纯化方法，并验证其通过激活受体CRLR/RAMP3复合物的生物活性，为后续功能研究奠定基础。
2. **"Recombinant ADM2 Attenuates Myocardial Ischemia-Reperfusion Injury via Antioxidant Pathways"**
- 作者：Wang H, et al.
- 摘要：探讨重组ADM2蛋白在心肌缺血再灌注损伤模型中的保护作用，发现其通过抑制氧化应激和炎症反应改善心脏功能。
3. **"ADM2 Recombinant Protein Regulates Angiogenesis in Endothelial Cells"**
- 作者：Suzuki Y, et al.
- 摘要：研究证明重组ADM2通过激活PI3K/Akt信号通路促进内皮细胞迁移和血管生成，提示其在血管修复中的潜在应用价值。
4. **"Structural and Functional Analysis of Proadrenomedullin N-Terminal 20 Peptide (PAMP-20) Derived from ADM2 Precursor"**
- 作者：Zhang Q, et al.
- 摘要：通过重组技术获得ADM2前体衍生的PAMP-20肽段，解析其结构并揭示其调控血压和能量代谢的分子机制。
(注：以上文献信息为模拟示例，实际研究中请根据具体需求检索真实文献。)
**Background of ADM2 Recombinant Protein**
ADM2 (Adrenomedullin 2), also known as intermedin (IMD), is a peptide hormone belonging to the calcitonin gene-related peptide (CGRP) family. It was first identified in 2004 through genomic analyses, sharing structural homology with adrenomedullin (ADM) and CGRP. ADM2 is encoded by the *ADM2* gene and is widely expressed in various tissues, including the brain, cardiovascular system, kidneys, and gastrointestinal tract.
Functionally, ADM2 interacts with receptor complexes composed of calcitonin receptor-like receptor (CLR) and receptor activity-modifying proteins (RAMPs), primarily RAMP1 and RAMP3. This interaction activates downstream signaling pathways, such as cAMP/PKA and MAPK, regulating vasodilation, anti-inflammatory responses, and cellular proliferation. ADM2 plays critical roles in maintaining cardiovascular homeostasis, fluid balance, and metabolic regulation. It exhibits protective effects in conditions like hypertension, heart failure, and ischemia-reperfusion injury by improving blood flow and reducing oxidative stress.
The recombinant ADM2 protein is produced using biotechnological platforms (e.g., *E. coli* or mammalian cell systems) to ensure high purity and bioactivity. Its therapeutic potential is being explored in preclinical studies for treating cardiovascular diseases, metabolic disorders, and inflammatory conditions. However, challenges such as peptide stability, delivery efficiency, and receptor specificity require further optimization. Research on ADM2 continues to unravel its multifaceted roles in physiology and pathology, highlighting its promise as a diagnostic marker or therapeutic target.
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