关键信息
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基因名
TLR4
- 应用
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别名
CD284; TOLL; HToll
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种属
Goat
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表达系统
E. coli
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标签
N-His
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纯度
Greater than 90% as determined by reducing SDS-PAGE.
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蛋白编号
E5FXB8
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表达区间
Trp26~Leu253
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分子量
32kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
Toll-like receptor 4 (TLR4) plays a crucial role in the innate immune response by recognizing pathogen-associated molecular patterns, particularly lipopolysaccharides (LPS) from gram-negative bacteria. The study of TLR4 recombinant proteins has gained significant attention due to their potential in vaccine development, immunotherapy, and understanding immune system mechanisms. Given that TLR4 signaling is fundamental in mediating inflammatory responses, researchers are keen to explore the receptor's structure and function via recombinant technology. Producing TLR4 as a recombinant protein allows for detailed investigations into its binding interactions, signaling pathways, and downstream effects. It also promotes the development of novel therapeutic strategies to modulate immune responses in various diseases, including sepsis, autoimmune disorders, and cancer. Furthermore, TLR4's involvement in the pathogenesis of metabolic conditions, such as obesity and diabetes, underscores the importance of elucidating its functional properties. Overall, continued research on TLR4 recombinant proteins represents a promising avenue for advancing our understanding of immune regulation and for developing new immunotherapeutic interventions.
