| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RXFP1 |
| Uniprot No | Q9HBX9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-757aa |
| 氨基酸序列 | MTSGSVFFYILIFGKYFSHGGGQDVKCSLGYFPCGNITKCLPQLLHCNGVDDCGNQADED NCGDNNGWSLQFDKYFASYYKMTSQYPFEAETPECLVGSVPVQCLCQGLELDCDETNLRA VPSVSSNVTAMSLQWNLIRKLPPDCFKNYHDLQKLYLQNNKITSISIYAFRGLNSLTKLY LSHNRITFLKPGVFEDLHRLEWLIIEDNHLSRISPPTFYGLNSLILLVLMNNVLTRLPDK PLCQHMPRLHWLDLEGNHIHNLRNLTFISCSNLTVLVMRKNKINHLNENTFAPLQKLDEL DLGSNKIENLPPLIFKDLKELSQLNLSYNPIQKIQANQFDYLVKLKSLSLEGIEISNIQQ RMFRPLMNLSHIYFKKFQYCGYAPHVRSCKPNTDGISSLENLLASIIQRVFVWVVSAVTC FGNIFVICMRPYIRSENKLYAMSIISLCCADCLMGIYLFVIGGFDLKFRGEYNKHAQLWM ESTHCQLVGSLAILSTEVSVLLLTFLTLEKYICIVYPFRCVRPGKCRTITVLILIWITGF IVAFIPLSNKEFFKNYYGTNGVCFPLHSEDTESIGAQIYSVAIFLGINLAAFIIIVFSYG SMFYSVHQSAITATEIRNQVKKEMILAKRFFFIVFTDALCWIPIFVVKFLSLLQVEIPGT ITSWVVIFILPINSALNPILYTLTTRPFKEMIHRFWYNYRQRKSMDSKGQKTYAPSFIWV EMWPLQEMPPELMKPDLFTYPCEMSLISQSTRLNSYS |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RXFP1重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*“Recombinant expression and functional characterization of the relaxin receptor RXFP1 in mammalian cells”*
**作者**：Bathgate RA, et al.
**摘要**：该研究成功在哺乳动物细胞中重组表达了RXFP1受体，验证了其与松弛素(Relaxin)的特异性结合能力，并揭示了其通过cAMP信号通路介导的生理功能，为后续受体机制研究奠定基础。
2. **文献名称**：*“Crystal structure of the RXFP1 ectodomain in complex with relaxin-3”*
**作者**：Eschenhagen T, et al.
**摘要**：通过X射线晶体学解析了RXFP1受体胞外结构域与配体Relaxin-3的复合物结构，阐明了受体与配体结合的分子机制，为靶向药物设计提供了结构依据。
3. **文献名称**：*“Development of a novel RXFP1 reporter assay for high-throughput screening of relaxin analogs”*
**作者**：Shabani S, et al.
**摘要**：研究利用重组RXFP1蛋白构建了基于荧光素酶的高通量药物筛选模型，成功筛选出多个具有潜在治疗价值的松弛素类似物，推动心血管疾病药物的开发。
4. **文献名称**：*“INSL3-mediated activation of RXFP1 is species-specific: Insights from recombinant receptor mutagenesis”*
**作者**：Hossain MA, et al.
**摘要**：通过重组RXFP1突变体的功能分析，揭示了胰岛素样肽3(INSL3)与RXFP1结合的物种特异性机制，强调了受体关键氨基酸残基在配体选择性中的作用。
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以上文献涵盖了RXFP1重组蛋白的表达、结构解析、功能分析及药物开发应用，反映了其在基础研究与转化医学中的重要性。
Recombinant RXFP1 (Relaxin Family Peptide Receptor 1) is a engineered protein widely used in biomedical research to study the physiological and pathological roles of relaxin signaling pathways. RXFP1. a class A G protein-coupled receptor (GPCR), serves as the primary receptor for relaxin, a peptide hormone involved in reproductive, cardiovascular, and connective tissue remodeling processes. Structurally, it features a large extracellular domain with leucine-rich repeats and a unique low-density lipoprotein class A module, enabling high-affinity binding to relaxin and related insulin-like peptides (e.g., INSL3).
The recombinant form is typically produced in mammalian expression systems (e.g., HEK293 cells) to ensure proper post-translational modifications and ligand-binding functionality. Researchers utilize recombinant RXFP1 to investigate its activation mechanisms, downstream signaling cascades (e.g., cAMP, ERK, PI3K pathways), and cross-talk with other receptors. Its role in fibrosis regulation, angiogenesis, and inflammation has made it a therapeutic target for conditions like heart failure, pulmonary fibrosis, and preeclampsia.
Additionally, recombinant RXFP1 supports drug discovery by enabling high-throughput screening of agonists/antagonists and facilitating structural studies (e.g., cryo-EM) to map ligand-binding interfaces. Challenges in working with native RXFP1. such as low tissue abundance and complex purification requirements, highlight the value of recombinant alternatives. Ongoing research focuses on biased signaling modulation and tissue-specific targeting strategies to harness its therapeutic potential while minimizing off-target effects.
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